Department of Pharmacology and Toxicology, Acharya & B.M. Reddy College of Pharmacy, Bangalore, Karnataka, India.
Phytomedicine. 2008 Nov;15(11):959-70. doi: 10.1016/j.phymed.2008.05.005. Epub 2008 Jul 7.
The neuropharmacological activities of the methanolic and aqueous extract of Wedelia calendulacea stem were screened in rats and mice. The extracts effect on pentobarbital-induced sleeping time, pentylenetetrazole- and styrychnine-induced seizure, spontaneous motor activity, exploratory behaviour, and rota-rod performance (motor coordination) were evaluated. The methanolic extract (20 and 50 mg/kg, i.p.) and aqueous extract (200 and 500 mg/kg, i.p.) produced a significant (p<0.001) prolongation of pentobarbital-induced sleeping time, and reduced the SMA and exploratory behaviour. The extract prolonged onset of the phases of seizure activity but did not protect mice against lethality induced by pentylenetetrazole and strychnine. It also failed to affect the motor coordination test. These results suggest that the extract contained an agent with neuropharmacological activity that may be sedative in nature. In addition, from the crude methanolic extract of Wedelia calendulacea stem a HPLC fingerprint profile and liquid chromatography/sequential mass spectrometry (LC/MS) were performed.
对黄花倒水莲茎的甲醇和水提取物的神经药理学活性在大鼠和小鼠中进行了筛选。评估了提取物对戊巴比妥钠诱导的睡眠时间、戊四氮和士的宁诱导的惊厥、自发运动活动、探索行为和转棒性能（运动协调）的影响。甲醇提取物（20 和 50mg/kg，ip）和水提取物（200 和 500mg/kg，ip）显著延长戊巴比妥钠诱导的睡眠时间（p<0.001），并减少 SMA 和探索行为。提取物延长了惊厥活动各阶段的发作时间，但不能保护小鼠免受戊四氮和士的宁引起的致死作用。它也未能影响运动协调测试。这些结果表明，提取物中含有一种具有神经药理学活性的物质，可能具有镇静作用。此外，从黄花倒水莲茎的粗甲醇提取物中进行了高效液相色谱指纹图谱和液相色谱/顺序质谱（LC/MS）分析。
