Mermillod P, Lonergan P, Carolan C, Khatir H, Poulin N, Cognié Y
INRA, Physiologie de la Reproduction des Mammifères Domestiques, Nouzilly.
Contracept Fertil Sex. 1996 Jul-Aug;24(7-8):552-8.
In vitro maturation represents the first step towards the in vitro production of embryos in domestic species. This production is of great interest from both zootechnic and basic points of view. Beyond nuclear aspects of maturation (progression of meiosis to metaphase II) cytoplasmic aspects confer to oocytes their potential to be fertilized and to develop normally. The culture medium used for maturation could influence this competence. Furthermore, the origin of oocytes (physiologic and genetic status of the oocyte donor, characteristics of the follicle) could also be determinant. The improvement of in vitro maturation techniques will certainly require a better understanding of these different aspects. Additionally, the maintenance of the oocytes in meiotic block during a pre-maturation in vitro culture will allow them to complete the storage of molecules necessary for fertilization and development.
体外成熟是家畜体外胚胎生产的第一步。从畜牧学和基础研究的角度来看，这种生产方式都具有重要意义。除了成熟的核方面（减数分裂进展至中期II），细胞质方面赋予卵母细胞受精和正常发育的潜力。用于成熟培养的培养基可能会影响这种能力。此外，卵母细胞的来源（卵母细胞供体的生理和遗传状态、卵泡的特征）也可能起决定性作用。体外成熟技术的改进肯定需要更好地理解这些不同方面。此外，在体外预成熟培养期间将卵母细胞维持在减数分裂阻滞状态，将使它们能够完成受精和发育所需分子的储存。
