Santos-Montes A, Gonzalo-Lumbreras R, Izquierdo-Hornillos R
Departamento de Química Analítica, Facultad de Química, Universidad Complutense, Madrid, Spain.
J Chromatogr B Biomed Appl. 1995 Nov 3;673(1):27-33. doi: 10.1016/0378-4347(95)00253-f.
A reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous determination of cortisol and cortisone in human urine samples using methylprednisolone as the internal standard is described. The method involves the systematic use of isocratic mobile phases of water and methanol, acetonitrile or tetrahydrofuran and a reversed-phase Hypersil C18 column. A water-acetonitrile mixture used as the mobile phase proved to be the most adequate one for analyzing urine samples purified by solvent extraction. The proposed method is sensitive, reproducible and selective. It was applied to the determination of cortisol and cortisone in several human urine samples: healthy subjects, sportsmen before and/or after stress for doping control purposes, and patients with Cushing's syndrome.
描述了一种以甲泼尼龙为内标同时测定人尿样中皮质醇和可的松的反相高效液相色谱（HPLC）方法。该方法系统地使用水与甲醇、乙腈或四氢呋喃的等度流动相以及反相Hypersil C18柱。用作流动相的水 - 乙腈混合物被证明是分析经溶剂萃取纯化的尿样的最合适的流动相。所提出的方法灵敏、可重现且具有选择性。它被应用于测定几个人尿样中的皮质醇和可的松：健康受试者、出于兴奋剂检测目的在应激前后的运动员以及库欣综合征患者。
