De Petrocellis L, Orlando P, Gavagnin M, Ventriglia M, Cimino G, Di Marzo V
Istituo di Cibernetica, C.N.R., Viale Toiano 6, I-80072 Arco Felice, Naples, Italy.
Experientia. 1996 Sep 15;52(9):874-7. doi: 10.1007/BF01938873.
Five novel 1,2-sn-diacylglycerols with diterpenoid acyl moieties in the sn-1 position were isolated and characterized, together with the corresponding 1,3-sn-diacylglycerols, from three species of dorid nudibranchs molluscs. Their potent activity as morphogens in vivo in the Hydra tentacle regeneration assay and their parallel activity as activators of rat brain protein kinase C (PKC) in vitro are reported here. Our findings promote the use of these compounds as useful molecular probes for both in vivo and in vitro studies on the participation of PKC in cell development.
从三种海兔科裸鳃亚目软体动物中分离并鉴定出了5种新型的1,2-二酰基甘油，其sn-1位带有二萜酰基部分，同时还分离出了相应的1,3-二酰基甘油。本文报道了它们在水螅触手再生试验中作为体内形态发生原的强大活性，以及在体外作为大鼠脑蛋白激酶C（PKC）激活剂的类似活性。我们的研究结果促进了这些化合物作为有用的分子探针，用于PKC参与细胞发育的体内和体外研究。
