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通过简单的水选择性NOESY-HSQC实验快速估算15N标记蛋白质的相对酰胺质子交换率。

Rapid estimation of relative amide proton exchange rates of 15 N-labelled proteins by a straightforward water selective NOESY-HSQC experiment.

作者信息

Böckmann A, Penin F, Guittet E

机构信息

Laboratoire de RMN, ISCN-CNRS, Gif sur Yvette, France.

出版信息

FEBS Lett. 1996 Apr 1;383(3):191-5. doi: 10.1016/0014-5793(96)00243-8.

DOI:10.1016/0014-5793(96)00243-8
PMID:8925894
Abstract

A straightforward heteronuclear pseudo-3D NOESY-HSQC pulse sequence using radiation damping to selectively invert magnetization at the water frequency was developed to estimate the amide proton exchange rates in 15N-labelled proteins. The peak intensities in the resultant 2D spectrum allow a direct classification of amide proton exchange rates according to short (ms), intermediate (ms to s) or long (> or = s) residence times. This method was successfully used for the analysis of amide proton exchange rates in the 15N-labelled FruR DNA-binding domain and pertinent information about its dynamics was obtained.

摘要

开发了一种直接的异核伪三维NOESY-HSQC脉冲序列，利用辐射阻尼在水频率下选择性地反转磁化，以估计15N标记蛋白质中的酰胺质子交换率。所得二维谱中的峰强度允许根据短（毫秒）、中（毫秒至秒）或长（>或 = 秒）停留时间对酰胺质子交换率进行直接分类。该方法成功用于分析15N标记的FruR DNA结合结构域中的酰胺质子交换率，并获得了有关其动力学的相关信息。

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