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A study of protein-water exchange through the off-resonance ROESY experiment: application to the DNA-binding domain of AlcR.

作者信息

Birlirakis N, Cerdan R, Guittet E

机构信息

Laboratoire de RMN, ICSN-CNRS, Gif-sur-Yvette, France.

出版信息

J Biomol NMR. 1996 Dec;8(4):487-91. doi: 10.1007/BF00228149.

DOI:10.1007/BF00228149
PMID:9008364
Abstract

In this communication a new NMR experiment for the safe observation and quantification of water-protein exchange phenomena is presented. It combines a water-selective pulse, offering chemical shift-based separation, and the off-resonance ROESY dynamic filter, which permits the elimination of the unwanted intramolecular dipolar cross relaxation of protein protons. Moreover, pulsed field gradients are used for the suppression of radiation damping and the solvent signal. The straightforward incorporation of this sequence in heteronuclear experiments is demonstrated for the case of the DNA-binding domain of the alcohol regulator protein.

摘要

相似文献

1
A study of protein-water exchange through the off-resonance ROESY experiment: application to the DNA-binding domain of AlcR.
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2
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本文引用的文献

1
Selective excitation of intense solvent signals in the presence of radiation damping.在辐射阻尼存在的情况下选择性激发强溶剂信号。
J Biomol NMR. 1995 Jun;5(4):420-6. doi: 10.1007/BF00182286.
2
Suppression of radiation damping during selective excitation of the water signal: The WANTED sequence.选择性激发水信号时抑制辐射阻尼：WANTED 序列。
J Biomol NMR. 1996 Jul;8(1):87-92. doi: 10.1007/BF00198142.
3
Rapid estimation of relative amide proton exchange rates of 15 N-labelled proteins by a straightforward water selective NOESY-HSQC experiment.
采用带有快速异核单量子相干（FHSQC）检测方案的相位调制CLEAN化学交换（CLEANEX-PM）方法对水-酰胺质子交换率进行精确定量。
J Biomol NMR. 1998 Feb;11(2):221-6. doi: 10.1023/a:1008276004875.
4
Three-dimensional solution structure of beta cryptogein, a beta elicitin secreted by a phytopathogenic fungus Phytophthora cryptogea.β-隐地蛋白的三维溶液结构，β-隐地蛋白是由植物致病真菌隐地疫霉分泌的一种β-激发素。
Protein Sci. 1997 Nov;6(11):2279-84. doi: 10.1002/pro.5560061101.
通过简单的水选择性NOESY-HSQC实验快速估算15N标记蛋白质的相对酰胺质子交换率。
FEBS Lett. 1996 Apr 1;383(3):191-5. doi: 10.1016/0014-5793(96)00243-8.
4
Solution structure of a Lewis(x) analogue by off-resonance 1H NMR spectroscopy without use of an internal distance reference.
J Biomol NMR. 1996 Jul;8(1):23-35. doi: 10.1007/BF00198137.
5
Separation of intramolecular NOE and exchange peaks in water exchange spectroscopy using spin-echo filters.使用自旋回波滤波器在水交换光谱中分离分子内NOE和交换峰。
J Biomol NMR. 1996 Jan;7(1):77-82. doi: 10.1007/BF00190459.
6
Measurement of amide proton exchange rates and NOEs with water in 13C/15N-enriched calcineurin B.在富含13C/15N的钙调神经磷酸酶B中测量酰胺质子交换率及与水的核Overhauser效应（NOE）
J Biomol NMR. 1993 Nov;3(6):627-38. doi: 10.1007/BF00198368.
7
Improved sensitivity of HSQC spectra of exchanging protons at short interscan delays using a new fast HSQC (FHSQC) detection scheme that avoids water saturation.使用一种避免水饱和的新型快速异核单量子相干谱（FHSQC）检测方案，在短的扫描间隔延迟下提高了可交换质子的HSQC谱的灵敏度。
J Magn Reson B. 1995 Jul;108(1):94-8. doi: 10.1006/jmrb.1995.1109.
8
Correct intron splicing generates a new type of a putative zinc-binding domain in a transcriptional activator of Aspergillus nidulans.
FEBS Lett. 1991 Mar 11;280(1):11-6. doi: 10.1016/0014-5793(91)80193-7.
9
Protein hydration in aqueous solution.蛋白质在水溶液中的水合作用。
Science. 1991 Nov 15;254(5034):974-80. doi: 10.1126/science.1948083.
10
Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.用于水溶液单量子核磁共振光谱的梯度定制激发
J Biomol NMR. 1992 Nov;2(6):661-5. doi: 10.1007/BF02192855.