George V, Jesudason M V, John T J
Department of Microbiology, Christian Medical College & Hospital, Vellore.
Indian J Med Res. 1996 Jan;103:55-7.
The confirmation of the identity of Vibrio cholerae serogroup 01 and serogroup 0139 is usually done by slide agglutination tests using specific antisera. Antiserum to V. cholerae 01 is freely available but not antiserum to V. cholerae 0139, thus making specific identification of the latter more difficult. A modified CAMP (Christie Atkins and Muench - Paterson) test has been described as a possibility in the identification of V. cholerae 0139 and we have evaluated this on 197 strains of organisms including 48 V. cholerae 0139, 50 V. cholerae 01, 49 non-01, non-139 V. cholerae and 50 Aeromonas species. The test had a sensitivity of 98.9 per cent and a specificity of 92.9 per cent in the identification of these strains, when compared with faeces culture as the gold standard.
霍乱弧菌01血清群和0139血清群的鉴定通常采用使用特异性抗血清的玻片凝集试验。霍乱弧菌01的抗血清可免费获得，但霍乱弧菌0139的抗血清则不然，因此后者的特异性鉴定更加困难。一种改良的CAMP（克里斯蒂、阿特金斯和明奇-帕特森）试验已被描述为鉴定霍乱弧菌0139的一种可能方法，我们对197株菌株进行了评估，其中包括48株霍乱弧菌0139、50株霍乱弧菌01、49株非01、非139霍乱弧菌和50株气单胞菌属菌种。与粪便培养作为金标准相比，该试验在鉴定这些菌株时的灵敏度为98.9%，特异性为92.9%。
