Giono-Cerezo S, Rodríguez Angeles M G, Gutiérrez-Cogco L, Valdespino-Gómez J L
Laboratorio de Bacteriología Entérica, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México, D.F., Mexico.
Rev Latinoam Microbiol. 1994 Oct-Dec;36(4):243-51.
We made 52180 tests for isolation and identification of toxigenic V. cholerae O1 from rectal swabs and reference strains. We isolated 17.6% V. cholerae O1 strains in 1991, 43.5% in 1992 and 38.9% in 1993. The main serovar in 1991 was Inaba, whereas in 1993 a similar percentage was serovar Ogawa. The phenotype of V. cholerae strains was determined by hemolysis test, Voges-Proskauer test, polymyxin B resistance and phages 4 and 5 resistance. All of the mexican strains were El Tor. There were 2.9-0.75% hemolytic strains from 1991 to 1993, but they were negative when the test was made in tube with human erythrocytes. The resistotypes were performed in 24526 selected strains by Kirby-Bauer method and MIC tests. All of the strains were sensitive, except more than 100 strains isolated in Veracruz that were resistant to tetracycline and doxycycline. Detection of cholera toxin was made by ELISA and on culture of Vero and CHO cells. All the V. cholerae O1 strains were toxigenic. The genotype was determined by PCR and ribotyping. The PCR amplified one 564 pb fragment on V. cholerae O1. The ribotypes of mexican strains were 5 and 6a.
我们对直肠拭子和参考菌株进行了52180次产毒霍乱弧菌O1的分离和鉴定试验。1991年，我们分离出17.6%的霍乱弧菌O1菌株，1992年为43.5%，1993年为38.9%。1991年的主要血清型是稻叶型，而1993年小川型的比例与之相似。通过溶血试验、Voges-Proskauer试验、多粘菌素B耐药性以及噬菌体4和5耐药性来确定霍乱弧菌菌株的表型。所有墨西哥菌株均为埃尔托生物型。1991年至1993年有2.9 - 0.75%的溶血菌株，但用人红细胞在试管中进行试验时它们呈阴性。通过Kirby-Bauer法和MIC试验对24526株选定菌株进行了耐药型检测。除在韦拉克鲁斯分离出的100多株对四环素和强力霉素耐药的菌株外，所有菌株均敏感。通过ELISA以及在Vero细胞和CHO细胞培养物上检测霍乱毒素。所有霍乱弧菌O1菌株均产毒。通过PCR和核糖体分型确定基因型。PCR在霍乱弧菌O1上扩增出一个564 pb的片段。墨西哥菌株的核糖体分型为5型和6a型。
