Humphrey M F, Moore S R
Lions Eye Institute, University of Western Australia, Nedlands.
Glia. 1995 Dec;15(4):367-76. doi: 10.1002/glia.440150402.
Nucleoside diphosphatase (NDP-ase) labelled microglial cells were examined in retinas of three strains of rabbit, the New Zealand White (albino), the Canberra Half-Lop, and the Dutch Belted. The distribution in the nerve fibre and inner plexiform layers was similar in all strains. However, the outer plexiform layer (OPL) of Dutch-Belted rabbits was completely covered with a population of non-overlapping NDP-ase positive microglia while the OPL of New Zealand White and Half-Lop strains contained only occasional isolated cells. In the Dutch-Belted strain the area covered by the processes of each cell was less in central than peripheral retina where the cell density was lower. In the central retina of the New Zealand White and Half-Lop strains the cells covered an area similar to that of peripheral Dutch-Belted cells, suggesting that they were at a low density and there was no hidden population of cells. This finding was confirmed by Griffonia simplicifolia lectin labelling. Therefore the data is consistent with there being a strain variation in OPL microglia. The intensity of NDP-ase label in the IPL and GCL was less in the New Zealand White and Half-Lop strains and although the intensity increased after retinal injury it never reached that of the Dutch-Belted retinas. These variations in the intensity of NDP-ase expression and the localization of microglial cells may be important in inflammation and also for CNS function.
在三种兔的视网膜中检查了核苷二磷酸酶（NDP-ase）标记的小胶质细胞，这三种兔分别是新西兰白兔（白化病）、堪培拉半垂耳兔和荷兰带兔。所有品系在神经纤维层和内丛状层的分布相似。然而，荷兰带兔的外丛状层（OPL）完全被一群不重叠的NDP-ase阳性小胶质细胞覆盖，而新西兰白兔和半垂耳兔品系的OPL中仅偶尔有孤立的细胞。在荷兰带兔品系中，每个细胞的突起覆盖的面积在视网膜中央比周边小，周边的细胞密度较低。在新西兰白兔和半垂耳兔品系的中央视网膜中，细胞覆盖的面积与荷兰带兔周边细胞的面积相似，这表明它们的密度较低，不存在隐藏的细胞群体。这一发现通过简单叶豆凝集素标记得到证实。因此，数据表明OPL小胶质细胞存在品系差异。新西兰白兔和半垂耳兔品系的内丛状层（IPL）和神经节细胞层（GCL）中NDP-ase标记的强度较低，尽管视网膜损伤后强度增加，但从未达到荷兰带兔视网膜的强度。NDP-ase表达强度和小胶质细胞定位的这些差异可能在炎症以及中枢神经系统功能中具有重要意义。
