Bartold P M, Raben A
Department of Dentistry, University of Queensland, Brisbane, Australia.
J Periodontal Res. 1996 Apr;31(3):205-16. doi: 10.1111/j.1600-0765.1996.tb00485.x.
Growth factors are potent bioactive molecules responsible for the co-ordination of many cells functions and interactions. Of these agents PDGF and IGF have shown particular promise as agents which may be used to stimulate periodontal regeneration. In order to further understand the mechanisms by which growth factors may work, a simple model of in vitro wound healing has been utilized to assess the effects of PDGF on human periodontal ligament fibroblasts and its potential to stimulate wound healing. Human periodontal ligament fibroblasts were plated into 24-well plates and upon reaching confluence were wounded by creating uniform discoid lesions stripped of cells. The influence of various concentrations of PDGF on cell proliferation, cell migration and extracellular matrix synthesis was monitored. The results of this study indicated that in the presence of 10 ng/ml PDGF on 0.2% fetal calf serum, both cell proliferation and cell migration were significantly stimulated. In the wounded cultures, PDGF appeared to cause a moderate stimulation of proteoglycan synthesis compared to unwounded cultures. In conclusion, the model system tested appears to be useful for studying fundamental cellular and biochemical events associated with wound healing. The effects of PDGF in this system confirm that it is capable of modulating fibroblasts in a manner compatible with the events associated with wound repair.
生长因子是强效生物活性分子，负责协调多种细胞功能和相互作用。在这些因子中，血小板衍生生长因子（PDGF）和胰岛素样生长因子（IGF）作为可用于刺激牙周组织再生的因子已显示出特别的前景。为了进一步了解生长因子发挥作用的机制，已利用一种简单的体外伤口愈合模型来评估PDGF对人牙周膜成纤维细胞的影响及其刺激伤口愈合的潜力。将人牙周膜成纤维细胞接种到24孔板中，细胞汇合后，通过制造去除细胞的均匀盘状损伤来造成伤口。监测不同浓度的PDGF对细胞增殖、细胞迁移和细胞外基质合成的影响。本研究结果表明，在含有10 ng/ml PDGF的0.2%胎牛血清中，细胞增殖和细胞迁移均受到显著刺激。在有伤口的培养物中，与无伤口的培养物相比，PDGF似乎对蛋白聚糖合成有适度刺激作用。总之，所测试的模型系统似乎有助于研究与伤口愈合相关的基本细胞和生化事件。PDGF在该系统中的作用证实，它能够以与伤口修复相关事件相适应的方式调节成纤维细胞。
