Shimoda A, Hanaumi K, Kumagai K
Immunological Research Section of Clinical Laboratory, Sendai Shakaihoken Hospital.
Tohoku J Exp Med. 1995 Dec;177(4):327-35. doi: 10.1620/tjem.177.327.
We found a TNF inhibitory factor with a molecular weight of 5 to 10 kDa in the human sera. The activity was detected by inhibiting the activity of serum to TNF-induced cytotoxicity against target cells. It was found in sera of all the healthy donors tested without any febrile diseases. Moreover, our results demonstrated that TNF inhibitory factor decreases in the semum of patients on regular hemodialysis treatment and in the serum of diabetes mellitus patients. The activity found in human sera was eluted from DEAE-cellulose column (Mono Q) at 0.25 and 0.45 M NaCl, and was labile to incubation for 60 min at 56 degrees C and susceptible to treatment with trypsin, which destroyed 60% of its biological activity. TNF inhibitory factor may act as a regulator of the biological activity of TNF and could have beneficial effects in certain inflammatory conditions, and therefore, could be useful in clinical application.
我们在人血清中发现了一种分子量为5至10 kDa的肿瘤坏死因子（TNF）抑制因子。通过抑制血清对TNF诱导的针对靶细胞的细胞毒性活性来检测其活性。在所有接受检测的无任何发热性疾病的健康供者血清中均发现了该因子。此外，我们的结果表明，定期血液透析治疗患者的血清以及糖尿病患者的血清中TNF抑制因子含量降低。在人血清中发现的活性物质在0.25和0.45 M NaCl浓度下从DEAE-纤维素柱（Mono Q）上洗脱下来，在56℃孵育60分钟后不稳定，并且对胰蛋白酶处理敏感，胰蛋白酶可破坏其60%的生物活性。TNF抑制因子可能作为TNF生物活性的调节剂，在某些炎症状态下可能具有有益作用，因此，可能在临床应用中有用。
