Umehara F, Izumo S, Takeya M, Takahashi K, Sato E, Osame M
Third Department of Internal Medicine, Kagoshima University School of Medicine, Japan.
Acta Neuropathol. 1996;91(4):343-50. doi: 10.1007/s004010050435.
Leukocyte adhesion molecules to endothelium plays an important role in the pathogenesis of inflammatory diseases, including HTLV-I-associated myelopathy (HAM)/tropical spastic paraparesis (TSP). To help define the role of adhesion molecules in HAM/TSP, we studied the expression of lymphocyte function-associated antigen-1 (LFA-1), Mac-1, very late antigen-4 (VLA-4), Sialyl Lewisx (SLex), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), endothelial leukocyte adhesion molecule-1 (ELAM-1) and monocyte chemoattractant protein-1 (MCP-1) in the spinal cord lesions of HAM/TSP. The results indicate that spinal cord lesions of HAM/TSP have greater VCAM-1 expression on endothelium compared with those of controls. Infiltrating mononuclear cells, especially perivascular lesions, expressed VLA-4. Although the expression of ICAM-1 in the spinal cords was not distinctive between HAM/TSP and controls, infiltrating mononuclear cells in the spinal cords of HAM/TSP strongly expressed LFA-1 and Mac-1. ELAM-1 was expressed on endothelium in the inactive-chronic lesions from three of five HAM/HAM/TSP, but was not detectable in the spinal cords of controls. SLex reactions was detectable on occasional perivascular cells in the spinal cord of HAM/TSP, but not in those controls. MCP-1 was detectable on perivascular infiltrating cells and vascular endothelium in active-chronic lesions. This study suggest that VLA-4/VCAM-1 interaction may play an important role for lymphocyte migration into the central nervous system (CNS) and MCP-1 may also be involved in inflammatory cell recruitment to the CNS in HAM/TSP.
白细胞与内皮细胞的黏附分子在包括人类嗜T淋巴细胞病毒I型相关脊髓病（HAM）/热带痉挛性截瘫（TSP）在内的炎症性疾病发病机制中起重要作用。为了明确黏附分子在HAM/TSP中的作用，我们研究了淋巴细胞功能相关抗原-1（LFA-1）、巨噬细胞抗原-1（Mac-1）、极迟抗原-4（VLA-4）、唾液酸化路易斯寡糖x（SLex）、细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）、内皮细胞白细胞黏附分子-1（ELAM-1）和单核细胞趋化蛋白-1（MCP-1）在HAM/TSP脊髓病变中的表达。结果表明，与对照组相比，HAM/TSP脊髓病变内皮细胞上VCAM-1表达更高。浸润的单核细胞，尤其是血管周围病变中的单核细胞，表达VLA-4。尽管HAM/TSP和对照组脊髓中ICAM-1的表达无明显差异，但HAM/TSP脊髓中浸润的单核细胞强烈表达LFA-1和Mac-1。ELAM-1在五例HAM/TSP中三例的静止-慢性病变的内皮细胞上表达，但在对照组脊髓中未检测到。SLex反应在HAM/TSP脊髓中偶尔的血管周围细胞上可检测到，但在对照组中未检测到。MCP-1在活动-慢性病变的血管周围浸润细胞和血管内皮上可检测到。本研究提示，VLA-4/VCAM-1相互作用可能在淋巴细胞迁移至中枢神经系统（CNS）中起重要作用，且MCP-1也可能参与HAM/TSP中炎症细胞向CNS的募集。
