Mashige F, Matsushima Y, Kanazawa H, Sakuma I, Takai N, Bessho F, Ohkubo A
Department of Laboratory Medicine, University of Tokyo, Japan.
Ann Clin Biochem. 1996 Jan;33 ( Pt 1):43-9. doi: 10.1177/000456329603300106.
Concentrations of vanillylmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), vanillic acid (VA) and 5-hydroxyindoleacetic acid (5-HIAA) in urine from healthy subjects were determined by a high-performance liquid chromatography system with a mixed-mode (C18/anion-exchange) column and an 8-channel electrochemical detector, in order to study the influence of diet, diurnal variation and age. The urinary excretion of 5-HIAA increased significantly after eating banana, pineapple, tomato, kiwi fruit and walnut. An increase in the urinary excretion of DOPAC and HVA after eating banana and that of VA after taking vanilla was also noted. The urinary excretion of VMA was not significantly influenced by any of the foods examined. The urinary excretion of 5-HIAA in the first-morning urine increased beyond the upper limit of the reference value when banana was taken at 2000 h the previous day. The excretion of all metabolites in the second-morning urine in the fasting state was within respective reference ranges. Diurnal variation of the excretion of VMA, DOPAC, HVA and 5-HIAA in urine was relatively small, but that of VA was large. The concentrations (mmol/mol creatinine) of VMA, DOPAC, HVA, 5-HIAA and VA in the first-morning urine from healthy subjects increased from 7 days after birth to 1 year and then decreased to adult levels at 13 years of age.
采用配备混合模式（C18/阴离子交换）柱和8通道电化学检测器的高效液相色谱系统，测定健康受试者尿液中香草扁桃酸（VMA）、3,4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）、香草酸（VA）和5-羟色胺酸（5-HIAA）的浓度，以研究饮食、昼夜变化和年龄的影响。食用香蕉、菠萝、番茄、猕猴桃和核桃后，5-HIAA的尿排泄量显著增加。还注意到食用香蕉后DOPAC和HVA的尿排泄量增加，食用香草后VA的尿排泄量增加。VMA的尿排泄量不受任何所检测食物的显著影响。如果前一天20:00食用香蕉，晨尿中5-HIAA的尿排泄量会超过参考值上限。禁食状态下第二天晨尿中所有代谢物的排泄量均在各自的参考范围内。尿液中VMA、DOPAC、HVA和5-HIAA排泄量的昼夜变化相对较小，但VA的昼夜变化较大。健康受试者晨尿中VMA、DOPAC、HVA、5-HIAA和VA的浓度（mmol/mol肌酐）从出生后7天到1岁增加，然后在13岁时降至成人水平。
