Hutter H, Hammer A, Blaschitz A, Hartmann M, Ebbesen P, Dohr G, Ziegler A, Uchanska-Ziegler B
Institut für Histologie und Embryologie, Karl-Franzens-Universität, Graz, Austria.
Cell Tissue Res. 1996 Dec;286(3):439-47. doi: 10.1007/s004410050713.
Expression of HLA class I molecules in trophoblast cells from various locations in normal human first trimester and term placenta was investigated by immunohistochemistry with a panel of monoclonal antibodies against the heavy chains or complete HLA class I molecules complexed with beta2-microglobulin. These reagents were also employed to distinguish between the products of different HLA class I loci. In addition to previously characterized reagents, a novel monoclonal antibody against HLA-A molecules (TU155) was used. Various choriocarcinoma and transfected cell lines served as controls for the specificities of the monoclonal antibodies. Cells in close contact with maternal cells, such as invading trophoblast cells and cells of the basal plate, expressed beta2-m micro globulin in association with HLA-G and HLA-C heavy chains. These class I heavy chains may also have been present as isolated molecules, although not in each of the cells. In contrast, cells of the chorion laeve exclusively expressed HLA-G, and not HLA-A, -B, or -C antigens. Our data support the often discussed immune protective function and the regulatory function of the HLA-G molecule, during invasion. In addition, by using monoclonal antibodies HCA2 (anti-HLA-A and -G), HC10 (anti-HLA-B and -C), TU149 (anti-HLA-B, -C, and some -A alleles), SFR8-B6 (anti-HLA-Bw6 and some -C), LA45 (some HLA-A and -B), TU48 (anti-HLA-Bw4 and some -A), and TU155 (anti-HLA-A), we show the presence of HLA-C molecules in all extravillous trophoblast cells of the cell columns and in the basal plate; the trophoblast cells of the chorion laeve lack this antigen. The function of this molecule is not clear, although a protective function against natural killer cell activity in the endometrium is postulated.
采用一组针对重链或与β2-微球蛋白复合的完整HLA I类分子的单克隆抗体，通过免疫组织化学方法研究了正常人类早孕期和足月胎盘不同部位滋养层细胞中HLA I类分子的表达情况。这些试剂还用于区分不同HLA I类基因座的产物。除了先前已鉴定的试剂外，还使用了一种针对HLA-A分子的新型单克隆抗体（TU155）。各种绒毛膜癌和转染细胞系用作单克隆抗体特异性的对照。与母体细胞紧密接触的细胞，如侵入性滋养层细胞和基底板细胞，表达与HLA-G和HLA-C重链相关的β2-微球蛋白。这些I类重链也可能以分离分子的形式存在，尽管并非每个细胞都有。相比之下，平滑绒毛膜细胞仅表达HLA-G，而不表达HLA-A、-B或-C抗原。我们的数据支持了HLA-G分子在侵入过程中经常被讨论的免疫保护功能和调节功能。此外，通过使用单克隆抗体HCA2（抗HLA-A和-G）、HC10（抗HLA-B和-C）、TU149（抗HLA-B、-C和一些-A等位基因）、SFR8-B6（抗HLA-Bw6和一些-C）、LA45（一些HLA-A和-B）、TU48（抗HLA-Bw4和一些-A）和TU155（抗HLA-A），我们发现细胞柱和基底板的所有绒毛外滋养层细胞中都存在HLA-C分子；平滑绒毛膜的滋养层细胞缺乏这种抗原。尽管推测该分子对子宫内膜中的自然杀伤细胞活性具有保护作用，但其功能尚不清楚。
