Cifuentes M, López-Avalos M D, Pérez J, Grondona J M, Fernández-Llebrez P
Departamento de Biología Animal, Facultad de Ciencias, Universidad de Málaga, Málaga E-29071, Spain.
Cell Tissue Res. 1996 Dec;286(3):543-6. doi: 10.1007/s004410050724.
The subcommissural organ is an ependymal brain gland that secretes, into the ventricular cerebrospinal fluid, high molecular weight glycoproteins that form Reissner's fiber. Precursor and processed forms of secretion have been demonstrated by immunoblotting in the subcommissural organ of mammals and fish. In the chicken only a processed form has as yet been identified. In the present report, we have studied the subcommissural organ of 13-day-old chick embryos using (1) an antiserum against bovine Reissner's fiber, and (2) the lectins, concanavalin A and Limax flavus agglutinin. Paraffin sections of the subcommissural organ and blots of subcommissural organ extracts have been analyzed. The ependymal cells of sectioned subcommissural organ are strongly stained with the antiserum. Concanavalin A binds to materials in all cytoplasmatic regions, whereas Limax flavus agglutinin identifies materials confined to the apex of the ependymal cells. In the blots, a band of 540 kDa is immunostained. This band is positive for concanavalin A positive but negative for Limax flavus agglutinin and is thereby regarded as representing a precursor form of the secretion.
连合下器官是一种室管膜脑腺，它向脑室脑脊液中分泌形成赖氏纤维的高分子量糖蛋白。在哺乳动物和鱼类的连合下器官中，已通过免疫印迹法证实了分泌物的前体形式和加工形式。在鸡中，目前仅鉴定出一种加工形式。在本报告中，我们使用（1）抗牛赖氏纤维抗血清和（2）凝集素刀豆球蛋白A和黄斑海兔凝集素，研究了13日龄鸡胚的连合下器官。分析了连合下器官的石蜡切片和连合下器官提取物的印迹。切片的连合下器官的室管膜细胞被抗血清强烈染色。刀豆球蛋白A与所有细胞质区域的物质结合，而黄斑海兔凝集素识别局限于室管膜细胞顶端的物质。在印迹中，一条540 kDa的条带被免疫染色。这条带对刀豆球蛋白A呈阳性，但对黄斑海兔凝集素呈阴性，因此被认为代表分泌物的前体形式。
