Charpin C, Garcia S, Bouvier C, Andrac L, Devictor B, Lavaut M N, Allasia C, Bonnier P, Piana L
Department of Pathology, Hôpital Nord, Marseille, France.
Am J Clin Pathol. 1996 Nov;106(5):640-6. doi: 10.1093/ajcp/106.5.640.
A series of 222 tumor samples stored at -80 degrees C in the authors' tumor library were investigated with anti-p53 (PA 1801) and streptavidin-biotin-peroxidase complex. The p53 immunoprecipitates were quantified by densitometry assessed by image analysis of digitized microscopic images. Two parameters, percentage of positive surface and mean optical densities, were compared with the patient's outcome (follow-up = 96.8 months) (life table method, Mantel Cox test, BMDP statistical software). The p53 expression significantly correlated with a poor overall survival (P = .0063), metastasis-free survival (P = .024), and recurrence-free survival (P = .022) at a 20% cutoff point of positive immunoreactive tumor surface. A strong prognostic significance was observed in the node-positive subset of patients but not in the node-negative subset, except for recurrence-free survival (P = .047). The results indicate the clinical relevance p53 evaluated by quantitative immunocytochemistry.
作者肿瘤库中保存在-80℃的222份肿瘤样本，采用抗p53（PA 1801）和链霉亲和素-生物素-过氧化物酶复合物进行研究。通过对数字化显微图像的图像分析进行光密度测定，对p53免疫沉淀物进行定量分析。将两个参数，即阳性表面百分比和平均光密度，与患者的预后（随访时间=96.8个月）进行比较（寿命表法、Mantel Cox检验、BMDP统计软件）。在阳性免疫反应性肿瘤表面的20%截断点处，p53表达与总体生存率差（P = 0.0063）、无转移生存率（P = 0.024）和无复发生存率（P = 0.022）显著相关。除无复发生存率外（P = 0.047），在淋巴结阳性患者亚组中观察到了较强的预后意义，但在淋巴结阴性亚组中未观察到。结果表明通过定量免疫细胞化学评估p53具有临床相关性。
