Gonzalez-Palacios F, Sancho M, Martinez J C, Bellas C
Department of Pathology, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, Spain.
Mol Pathol. 1997 Dec;50(6):304-9. doi: 10.1136/mp.50.6.304.
To investigate the possibility of a correlation among microvessel density, p53 overexpression, and apoptosis in invasive breast carcinoma.
Microvessel density was analysed in 105 cases of invasive breast carcinoma by immunohistology using antifactor VIII related antibody. The results were correlated with the immunohistochemical expression of p53 and the apoptotic index, detected using the in situ end labelling of fragmented DNA method (TUNEL). Assessment was made with a CAS 200 image analyser. All these studies were performed on formalin fixed, paraffin wax embedded tissue sections of tumour samples.
The mean (SD) microvessel count was 47.2 (51.1), with a range from 7 to 250. Thirty five (33%) carcinomas showed overexpression of p53 protein. The apoptotic index of tumours ranged from 0.0 to 28.0, with a mean (SD) of 1.7 (3.2). The results showed that there was a significant inverse correlation between microvessel density and p53 expression (p = 0.04; odds ratio, 0.37). In contrast, no correlation was identified between the microvessel density and apoptotic index.
These results suggest that in invasive breast carcinoma the p53 overexpression phenotype downregulates tumour neoangiogenesis, as does the wild-type of p53 protein. In addition, they suggest that apoptosis and neoangiogenesis in these tumours are independent processes.
探讨浸润性乳腺癌中微血管密度、p53过表达与细胞凋亡之间存在相关性的可能性。
采用抗因子Ⅷ相关抗体，通过免疫组织化学方法对105例浸润性乳腺癌的微血管密度进行分析。将结果与p53的免疫组化表达以及凋亡指数相关联，凋亡指数采用DNA片段原位末端标记法（TUNEL）检测。使用CAS 200图像分析仪进行评估。所有这些研究均在肿瘤样本的福尔马林固定、石蜡包埋组织切片上进行。
微血管计数的平均值（标准差）为47.2（51.1），范围为7至250。35例（33%）癌显示p53蛋白过表达。肿瘤的凋亡指数范围为0.0至28.0，平均值（标准差）为1.7（3.2）。结果显示微血管密度与p53表达之间存在显著负相关（p = 0.04；优势比，0.37）。相比之下，未发现微血管密度与凋亡指数之间存在相关性。
这些结果表明，在浸润性乳腺癌中，p53过表达表型与野生型p53蛋白一样，下调肿瘤新生血管生成。此外，这些结果表明这些肿瘤中的细胞凋亡和新生血管生成是独立的过程。
