Bugge T H, Kombrinck K W, Flick M J, Daugherty C C, Danton M J, Degen J L
Division of Developmental Biology, Children's Hospital Research Foundation, Cincinnati, Ohio 45229, USA.
Cell. 1996 Nov 15;87(4):709-19. doi: 10.1016/s0092-8674(00)81390-2.
Plasmin(ogen) is an extracellular serine protease implicated in the activation of latent growth factors and procollagenase, degradation of extracellular matrix components, and fibrin clearance. Plasminogen (Plg) deficiency in mice results in high mortality, wasting, spontaneous gastrointestinal ulceration, rectal prolapse, and severe thrombosis. Furthermore, Plg-deficient mice display delayed wound healing following skin injury, a defect partly related to impaired keratinocyte migration. We generated mice deficient in Plg and fibrinogen (Fib) and show that removal of fibrin(ogen) from the extracellular environment alleviates the diverse spontaneous pathologies previously associated with Plg deficiency and corrects healing times. Mice deficient in Plg and Fib are phenotypically indistinguishable from Fib-deficient mice. These data suggest that the fundamental and possibly only essential physiological role of Plg is fibrinolysis.
纤溶酶（原）是一种细胞外丝氨酸蛋白酶，与潜在生长因子和前胶原酶的激活、细胞外基质成分的降解以及纤维蛋白清除有关。小鼠纤溶酶原（Plg）缺乏会导致高死亡率、消瘦、自发性胃肠道溃疡、直肠脱垂和严重血栓形成。此外，Plg缺乏的小鼠在皮肤损伤后伤口愈合延迟，这一缺陷部分与角质形成细胞迁移受损有关。我们培育出了Plg和纤维蛋白原（Fib）缺乏的小鼠，并表明从细胞外环境中去除纤维蛋白（原）可减轻先前与Plg缺乏相关的各种自发性病理状况，并纠正愈合时间。Plg和Fib缺乏的小鼠在表型上与Fib缺乏的小鼠无法区分。这些数据表明，Plg的基本且可能唯一重要的生理作用是纤维蛋白溶解。
