Khaldoyanidi S, Denzel A, Zöller M
Department of Tumor Progression and Immune Defense, German Cancer Research Center, Heidelberg.
J Leukoc Biol. 1996 Nov;60(5):579-92. doi: 10.1002/jlb.60.5.579.
The hematopoietic form of the adhesion molecule CD44 is known to be involved in lymphocyte maturation and homing. To define lineage and stage of maturation, which requires expression of CD44, murine long-term bone marrow cultures were established. Stroma formation and proliferation of early as well as committed erythroid, myeloid, and lymphoid progenitors were evaluated under the influence of monoclonal anti-CD44 antibodies. Although the formation of stromal elements was not affected, formation of cobblestone areas was completely abolished. [3H]thymidine suicide confirmed that anti-CD44 treatment interfered with the proliferation of early and committed hematopoietic progenitor cells. In addition, homing and seeding of bone marrow cells was impaired by anti-CD44. The data are indicative of a dual functional mode of CD44 in adhesion and proliferation of hematopoietic progenitors and confirm an essential requirement for the molecule in early stages of hematopoiesis.
已知黏附分子CD44的造血形式参与淋巴细胞的成熟和归巢。为了确定需要CD44表达的成熟谱系和阶段，建立了小鼠长期骨髓培养体系。在单克隆抗CD44抗体的影响下，评估了早期以及定向红细胞、髓细胞和淋巴细胞祖细胞的基质形成和增殖情况。虽然基质成分的形成未受影响，但鹅卵石区域的形成完全被消除。[3H]胸腺嘧啶核苷自杀实验证实，抗CD44处理会干扰早期和定向造血祖细胞的增殖。此外，抗CD44会损害骨髓细胞的归巢和播种。这些数据表明CD44在造血祖细胞的黏附和增殖中具有双重功能模式，并证实了该分子在造血早期阶段的基本需求。
