Tomita Y, Koizumi T, Mizuno K, Tokuhisa T
Department of Orthopedic Surgery, Kobe University School of Medicine.
Kobe J Med Sci. 1995 Oct;41(5):141-53.
Constitutive expression of the c-fos gene perturbs the de novo synthesis of RNA and DNA in B cells stimulated by surface immunoglobulin (sIg) cross-linking. In order to examine kinetics of the regulatory effect on the activation process of B cells, we used splenic B cells from transgenic mice carrying the c-fos gene under the control of the interferon alpha/beta (IFN)-inducible Mx gene promoter (Mx-c-fos). In the absence of IFN, Mx-c-fos B cells proliferated well by anti-IgM stimulation. However, both RNA and DNA synthesis in the Mx-c-fos B cells were markedly reduced by the addition of IFN in the culture within 12 h after anti-IgM stimulation. These results suggest that this regulatory effect of c-Fos displays at the mid G1 phase of the cell cycle.
c-fos基因的组成型表达会干扰表面免疫球蛋白（sIg）交联刺激的B细胞中RNA和DNA的从头合成。为了研究对B细胞激活过程的调节作用动力学，我们使用了来自转基因小鼠的脾B细胞，这些小鼠携带在干扰素α/β（IFN）诱导型Mx基因启动子（Mx-c-fos）控制下的c-fos基因。在没有IFN的情况下，Mx-c-fos B细胞通过抗IgM刺激增殖良好。然而，在抗IgM刺激后12小时内，向培养物中添加IFN会使Mx-c-fos B细胞中的RNA和DNA合成均显著减少。这些结果表明，c-Fos的这种调节作用在细胞周期的G1中期表现出来。
