Ng J, Hurley C K, Carter C, Baxter-Lowe L A, Bing D, Chopek M, Hegland J, Lee T D, Li T C, Hsu S, KuKuruga D, Mason J M, Monos D, Noreen H, Rosner G, Schmeckpeper B, Dupont B, Hartzman R J
Naval Medical Research Institute, Bethesda, Maryland, USA.
Tissue Antigens. 1996 Jan;47(1):21-6. doi: 10.1111/j.1399-0039.1996.tb02510.x.
DNA typing of HLA class II alleles of the DRB1/3/4/5 and DQB1 loci using sequence-specific oligonucleotide probes and polymerase chain reaction amplified DNA has been used for the large-scale typing of donors for the National Marrow Donor Program unrelated donor registry. The results of quality control analysis for the second year of the project (10/1/939/30/94) show the typing continues to be highly accurate, specific, and reliable. The average percent of correctly classified HLA oligotypes (groups of alleles defined by a hybridization pattern with a panel of sequence-specific oligonucleotide probes) based on 9,244 DRB1 and 7,244 DQB1 assignments was 99.8% (range 99.4%100.0%) for DRB1/DRB3/DRB4/DRB5 and 99.8% (range 99.6%100.0%) for DQB1. This level of accuracy is particularly remarkable because the 4,636 DRB quality control samples were tested blindly and could not be distinguished from 57,580 donor samples tested at the same time by the laboratories.
使用序列特异性寡核苷酸探针和聚合酶链反应扩增的DNA对DRB1/3/4/5和DQB1位点的HLA II类等位基因进行DNA分型，已用于国家骨髓捐赠计划无关供体登记处供体的大规模分型。该项目第二年（1993年10月1日至1994年9月30日）的质量控制分析结果表明，分型仍然高度准确、特异且可靠。基于9244个DRB1和7244个DQB1分型结果，DRB1/DRB3/DRB4/DRB5正确分类的HLA寡型（由一组序列特异性寡核苷酸探针的杂交模式定义的等位基因组）平均百分比为99.8%（范围99.4%至100.0%），DQB1为99.8%（范围99.6%至100.0%）。这种准确度水平尤其显著，因为4636个DRB质量控制样本是盲测的，无法与实验室同时检测的57580个供体样本区分开来。
