Cumberbatch M J, Herrero J F, Headley P M
Department of Physiology, School of Medical Sciences, University of Bristol, UK.
Neurosci Lett. 1996 Feb 2;204(1-2):33-6. doi: 10.1016/0304-3940(96)12309-0.
The (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA)/kainate receptor antagonists 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo[f]quinoxaline (NBQX) and 6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX) were examined by microiontophoretic administration in electrophysiological tests on spinal neurones in alpha-chloralose anaesthetized rats. The antagonists significantly reduced extracellularly recorded nociceptive and non-nociceptive responses, as expected; concurrently they reduced background discharge. When the background discharge rate was held constant, the antagonists no longer significantly reduced the evoked responses. This indicates that in the absence of such control, the antagonists decreased cell excitability and only indirectly affected the test responses. Unless such indirect effects have been controlled for, the interpretation of the actions of AMPA/kainate antagonists on evoked synaptic responses is compromised and may be erroneous.
在对α-氯醛糖麻醉大鼠的脊髓神经元进行的电生理测试中，通过微量离子电泳给药的方式，对（RS）-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）/红藻氨酸受体拮抗剂2,3-二羟基-6-硝基-7-氨磺酰基苯并[f]喹喔啉（NBQX）和6-氰基-7-硝基喹喔啉-2,3-二酮（CNQX）进行了检测。正如预期的那样，这些拮抗剂显著降低了细胞外记录的伤害性和非伤害性反应；同时，它们也降低了背景放电。当背景放电率保持恒定时，拮抗剂不再显著降低诱发反应。这表明，在没有这种控制的情况下，拮抗剂降低了细胞兴奋性，只是间接影响了测试反应。除非已经对这种间接效应进行了控制，否则对AMPA/红藻氨酸拮抗剂对诱发突触反应的作用的解释会受到影响，并且可能是错误的。
