Ishida N, Hasegawa T, Oyunsuren T, Mukoyama H
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Tokyo, Japan.
Anim Genet. 1996 Oct;27(5):359-63. doi: 10.1111/j.1365-2052.1996.tb00979.x.
The mitochondrial DNA sequence of cytochrome b gene in a Thoroughbred horse was determined. By comparing DNA sequences between the Thoroughbred and published sequence data (two horses and one Grevyi zebra), polymerase chain reaction (PCR) primers were designed for amplification of a 590 bp DNA fragment in the cytochrome b gene, and PCR-restriction fragment length polymorphism (RFLP) analysis was studied in 140 horses of six breeds using three restriction enzymes (AciI, BamHI, RsaI). Two morphs were found using each of the three enzymes. By combining three enzymes morphs, the 140 horses examined were classified into four types. Type 2 was most frequent in all breeds.
测定了一匹纯种马细胞色素b基因的线粒体DNA序列。通过比较该纯种马与已发表的序列数据（两匹马和一匹细纹斑马）之间的DNA序列，设计了聚合酶链反应（PCR）引物，用于扩增细胞色素b基因中590 bp的DNA片段，并使用三种限制性内切酶（AciI、BamHI、RsaI）对六个品种的140匹马进行了PCR-限制性片段长度多态性（RFLP）分析。使用这三种酶中的每一种都发现了两种形态。通过组合三种酶的形态，将所检测的140匹马分为四种类型。类型2在所有品种中最为常见。
