小鼠鼓索神经纤维神经元的咸味反应：存在两种对氯化钠敏感且温度依赖性不同的氨氯地平敏感受体成分的证据。

Salt taste responses of mouse chorda tympani neurons: evidence for existence of two different amiloride-sensitive receptor components for NaCl with different temperature dependencies.

Ninomiya Y

Department of Oral Physiology, Asahi University School of Dentistry, Gifu, Japan.

J Neurophysiol. 1996 Nov;76(5):3550-4. doi: 10.1152/jn.1996.76.5.3550.

Abstract

Inhibitory effects of amiloride on salt responses of single fibers of the chorda tympani nerve of the C57BL/6 strain of mice were examined at two different temperatures (approximately 12 and 24 degrees C). 2. Of 36 single fibers that responded to NaCl, 20 fibers showed strong suppression of responses to NaCl actuated by lingual treatment with amiloride (amiloride-sensitive fibers), whereas the remaining 16 fibers showed no such amiloride inhibition (amiloride-insensitive fibers). 3. Twenty amiloride-sensitive fibers were further classified into two subgroups according to the temperature dependency of their NaCl responses. In 15 of 20 fibers, amiloride-inhibitable NaCl responses were larger at 24 degrees C than at 12 degrees C, whereas the reverse was true for the remaining 5 fibers. All amiloride-insensitive fibers showed smaller responses to NaCl at 12 degrees C. 4. These results suggest that there exist two different amiloride-sensitive receptor components for NaCl with different temperature dependencies in mouse taste cells: one is more sensitive to NaCl at the higher temperature, and the other is more sensitive at the lower temperature.

摘要

在两个不同温度（约12摄氏度和24摄氏度）下，检测了氨氯地平对C57BL/6品系小鼠鼓索神经单纤维盐反应的抑制作用。2. 在对氯化钠有反应的36根单纤维中，20根纤维在用氨氯地平进行舌部处理后对氯化钠的反应受到强烈抑制（氨氯地平敏感纤维），而其余16根纤维未表现出这种氨氯地平抑制作用（氨氯地平不敏感纤维）。3. 根据对氯化钠反应的温度依赖性，将20根氨氯地平敏感纤维进一步分为两个亚组。在20根纤维中的15根中，氨氯地平可抑制的氯化钠反应在24摄氏度时比在12摄氏度时更大，而其余5根纤维则相反。所有氨氯地平不敏感纤维在12摄氏度时对氯化钠的反应较小。4. 这些结果表明，在小鼠味觉细胞中存在两种不同的对氯化钠敏感的受体成分，它们具有不同的温度依赖性：一种在较高温度下对氯化钠更敏感，另一种在较低温度下更敏感。