Bos R, van der Mei H C, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Eur J Oral Sci. 1996 Aug;104(4 ( Pt 1)):372-7. doi: 10.1111/j.1600-0722.1996.tb00094.x.
Coaggregation (interactions between two planktonic microorganisms) and co-adhesion (interactions between sessile and planktonic microorganisms) are believed to be important factors in the formation of dental plaque by many investigators, although others doubt whether coaggregation and co-adhesion occur in vivo. It is known that coaggregation and co-adhesion generally occur equally well in buffer as in saliva, but the influence of temperature on the co-adhesion of coaggregating oral microbial pairs in saliva is unknown. Therefore, co-adhesion of streptococci suspended in saliva to glass with adhering actinomyces present (1.0 x 10(6) cells cm-2) was studied in a parallel plate flow chamber in the temperature range from 22 degrees C to 40 degrees C. In the range from 22 degrees C up to 35 degrees C both pairs studied, Streptococcus oralis 34 with Actinomyces naeslundii 5951 and Streptococcus oralis J22 with A. naeslundii 5951, displayed similar co-adhesion kinetics and co-adhesion in a stationary end-point, but around and above 37 degrees C co-adhesion almost disappeared. Hence, we conclude that co-adhesion of coaggregating oral microbial pairs in saliva may be critically influenced by temperature, especially around the temperatures prevailing in the oral cavity.
许多研究人员认为，共聚集（两种浮游微生物之间的相互作用）和共黏附（固着微生物与浮游微生物之间的相互作用）是牙菌斑形成的重要因素，尽管也有其他人怀疑共聚集和共黏附是否在体内发生。已知共聚集和共黏附在缓冲液中与在唾液中通常表现得同样良好，但温度对唾液中共聚集的口腔微生物对的共黏附的影响尚不清楚。因此，在平行平板流动腔中研究了悬浮在唾液中的链球菌与存在黏附放线菌（1.0×10⁶ 个细胞/cm²）的玻璃之间在22℃至40℃温度范围内的共黏附情况。在22℃至35℃范围内，所研究的两对微生物，即口腔链球菌34与内氏放线菌5951以及口腔链球菌J22与内氏放线菌5951，在固定终点显示出相似的共黏附动力学和共黏附情况，但在37℃及以上时，共黏附几乎消失。因此，我们得出结论，唾液中共聚集的口腔微生物对的共黏附可能受到温度的关键影响，尤其是在口腔中普遍存在的温度附近。
