Suzuki S, Yamasaki K, Fujita T, Mamiya Y, Sonobe H
Biological Laboratory, Kanagawa Prefectural College, Yokohama, Japan.
Gen Comp Endocrinol. 1996 Nov;104(2):129-38. doi: 10.1006/gcen.1996.0155.
Ovarian and hemolymph ecdysteroids in Armadillidium vulgare were analyzed at four stages of ovarian maturation through the reproductive molt cycle using high-performance liquid chromatography and radioimmunoassay. The major ecdysteroids in the ovaries and hemolymph of A. vulgare were 20-hydroxyecdysone and ecdysone in free and conjugated forms. The concentration of ovarian ecdysteroids reached a maximal level in maturing ovaries during stage D (premolt period) of the molt cycle. At the end of stage D, a high level of ecdysteroids was detected in fully matured ovaries. On the other hand, hemolymph ecdysteroid titers in reproductive females showed a peak during stage D and declined rapidly to a low level at the end of stage D. No appreciable differences in the amounts of hemolymph ecdysteroids and in molecular species of them were apparent in females in reproductive and nonreproductive molt cycles. The amounts of hemolymph ecdysteroids were about fivefold higher in females than those in males. These ecdysteroids may have a role in controlling ovarian development in female A. vulgare.
利用高效液相色谱法和放射免疫分析法，在卵巢成熟的四个阶段，通过生殖蜕皮周期，对普通鼠妇卵巢和血淋巴中的蜕皮甾体进行了分析。普通鼠妇卵巢和血淋巴中的主要蜕皮甾体为游离形式和结合形式的20-羟基蜕皮酮和蜕皮酮。在蜕皮周期的D期（蜕皮前期），成熟卵巢中卵巢蜕皮甾体的浓度达到最高水平。在D期末，在完全成熟的卵巢中检测到高水平的蜕皮甾体。另一方面，生殖期雌性血淋巴中蜕皮甾体滴度在D期出现峰值，并在D期末迅速下降至低水平。在生殖和非生殖蜕皮周期的雌性中，血淋巴蜕皮甾体的含量及其分子种类没有明显差异。雌性血淋巴中蜕皮甾体的含量比雄性高约五倍。这些蜕皮甾体可能在控制普通鼠妇雌性的卵巢发育中发挥作用。
