Hilario E, Unda F, Pérez-Yarza G, Alvarez A, García-Sanz M, Allño S F
Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain.
Histol Histopathol. 1996 Oct;11(4):915-8.
The existence of cell surface-associated molecules has been claimed to play a major role in cellular recognition and interaction. In this respect, different tumor cell lines express laminin and its receptor, and this expression has been correlated with metastatic potential. In the present work, we have studied, by electron microscopic immunolabeling methods, the presence of laminin and 67KDa laminin-receptor on the surface of endothelial cells of lung blood capillaries. To label these molecules, we have developed an easy method in which the labeling is carried out "in situ", in previously excised lungs. The presence of both molecules was observed on the luminal surface of endothelial capillaries and, in many cases, gold particles were associated to small open vesicles of the endothelial cells. The results suggest that these molecules, traditionally associated to extracellular matrix, are also expressed in cellular surface of the lung vascular bed.
细胞表面相关分子的存在被认为在细胞识别和相互作用中起主要作用。在这方面，不同的肿瘤细胞系表达层粘连蛋白及其受体，并且这种表达与转移潜能相关。在本研究中，我们通过电子显微镜免疫标记方法研究了肺毛细血管内皮细胞表面层粘连蛋白和67kDa层粘连蛋白受体的存在情况。为了标记这些分子，我们开发了一种简便的方法，即在先前切除的肺中进行“原位”标记。在内皮毛细血管的管腔表面观察到了这两种分子的存在，并且在许多情况下，金颗粒与内皮细胞的小开放囊泡相关联。结果表明，这些传统上与细胞外基质相关的分子也在肺血管床的细胞表面表达。
