Hibma A M, Jassim S A, Griffiths M W
Department of Food Science, University of Guelph, Ont., Canada.
Int J Food Microbiol. 1996 Dec;33(2-3):157-67. doi: 10.1016/0168-1605(96)01123-3.
A bioluminescent phenotype of Listeria monocytogenes was employed to study the adhesion and biofilm formation of both classical and L-forms to relevant food and clinical contact surfaces. Attachment of both parental (cell-walled) and L-forms to intravenous tubing and stainless steel was compared using viable counts and bioluminescence. Both cell types attached themselves to intravenous tubing and stainless steel. Parental cell numbers attached to intravenous tubing were 1 log higher than L-form numbers, and on stainless steel, attached L-form numbers were 1 log higher. Bioluminescence measurements yielded a pattern similar to viable count measurements. There was a clear relationship between light output of attached bacteria and their viability and, thus, a bioluminescent phenotype provides a rapid monitor of both microbial viability and biofilm formation by L-forms.
利用单核细胞增生李斯特菌的生物发光表型来研究经典型和L型细菌对相关食品及临床接触表面的黏附及生物膜形成情况。通过活菌计数和生物发光法比较了亲代（细胞壁完整）细菌和L型细菌在静脉输液管和不锈钢表面的黏附情况。两种细胞类型均能黏附于静脉输液管和不锈钢表面。黏附于静脉输液管的亲代细胞数量比L型细菌多1个对数级，而在不锈钢表面，黏附的L型细菌数量比亲代细胞多1个对数级。生物发光测量结果与活菌计数测量结果相似。附着细菌的光输出与其活力之间存在明显关系，因此，生物发光表型可快速监测L型细菌的微生物活力和生物膜形成情况。
