Gao T, Lindholm T S, Marttinen A, Urist M R
Department of Clinical Medicine, University of Tampere, Finland.
Int Orthop. 1996;20(5):321-5. doi: 10.1007/s002640050086.
Few studies have been performed to investigate the characteristics of native Bone Morphogenetic Protein (BMP). We derived 5.85 g of native moose BMP from 102 kg of fresh moose bone and performed chromatographic studies to characterise its structure. We found the BMP molecule to have a molecular weight of 11-700 kDa. To investigate the ideal local delivery system for BMP we used porous discs as a carrier. These were implanted into 132 mice. The volume of new bone formed was evaluated by means of histological specimens, radiographs and isotope studies. The best results were obtained by implanting porous discs (coral and ceramic) with type IV collagen and BMP. We suggest that this is the best delivery system for future experiments regarding the acton of BMP.
很少有研究对天然骨形态发生蛋白（BMP）的特性进行调查。我们从102千克新鲜驼鹿骨中提取了5.85克天然驼鹿BMP，并进行了色谱研究以表征其结构。我们发现BMP分子的分子量为11 - 700 kDa。为了研究BMP的理想局部递送系统，我们使用多孔盘作为载体。将这些多孔盘植入132只小鼠体内。通过组织学标本、X光片和同位素研究来评估新形成骨的体积。通过植入含有IV型胶原蛋白和BMP的多孔盘（珊瑚和陶瓷）获得了最佳结果。我们认为这是未来关于BMP作用的实验的最佳递送系统。
