采用胶束电动色谱法检测细胞提取物中的蓝藻毒素（微囊藻毒素）。

Detection of cyanobacterial toxins (microcystins) in cell extracts by micellar electrokinetic chromatography.

作者信息

Bouaïcha N, Rivasseau C, Hennion M C, Sandra P

机构信息

CEMATMA, Laboratoire de Chimie Analytique, ESPCI, Paris, France.

出版信息

J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):53-7. doi: 10.1016/0378-4347(96)00128-4.

DOI:10.1016/0378-4347(96)00128-4

PMID:8930753

Abstract

A micellar electrokinetic chromatography (MEKC) method with UV detection is described for the rapid and efficient separation of three microcystins: microcystin-YR (MCYST-YR), microcystin-LR (MCYST-LR) and microcystin-RR (MCYST-RR). A detection limit of 7.5 pg for each toxin was achieved. The UV intensities of these toxins measured at 200 nm showed good linearity in the range 7.5-150 pg. The production of MCYST-LR in three cultured strains of cyanobacteria, namely Microcystis aeruginosa strain IP7806, Microcystis aeruginosa strain IP7813 and Oscillatoria agardhii strain IP7805, was evaluated.

摘要

本文描述了一种采用紫外检测的胶束电动色谱（MEKC）方法，用于快速高效地分离三种微囊藻毒素：微囊藻毒素-YR（MCYST-YR）、微囊藻毒素-LR（MCYST-LR）和微囊藻毒素-RR（MCYST-RR）。每种毒素的检测限为7.5 pg。这些毒素在200 nm处测得的紫外强度在7.5 - 150 pg范围内呈良好的线性关系。评估了三种蓝藻培养菌株，即铜绿微囊藻菌株IP7806、铜绿微囊藻菌株IP7813和阿氏颤藻菌株IP7805中MCYST-LR的产生情况。