Koganezawa M, Shingai R, Isono K, Shimada I
Biological Institute, Graduate School of Science, Tohoku University, Sendai, Japan.
Neuroreport. 1996 Sep 2;7(13):2063-7. doi: 10.1097/00001756-199609020-00001.
Functional expression of the gustatory sensitivity to amino acids and sugars was investigated in Xenopus oocytes injected with poly(A+) RNA extracted from fleshfly labellar taste organs. The current induced by the application of amino acids and sugars that stimulate fleshfly sugar receptor cells was recorded under voltage clamp conditions. L-Phenylalanine and L-valine induced a large transient inward current in poly(A+) RNA-injected oocytes but only small responses in uninjected control oocytes. Glucose and fructose also produced inward currents in the RNA-injected oocytes. N-methylated compounds or D-isomers of these amino acids induced either no response or a much smaller response than L-amino acids as they did in the fleshfly sugar receptor cells. The oocyte expression system is a useful tool for characterizing the taste transduction mechanism.
利用从肉蝇唇瓣味觉器官提取的聚腺苷酸（poly(A+)）RNA注射非洲爪蟾卵母细胞，研究了对氨基酸和糖类味觉敏感性的功能表达。在电压钳制条件下，记录了刺激肉蝇糖受体细胞的氨基酸和糖类所诱导的电流。L-苯丙氨酸和L-缬氨酸在注射了聚腺苷酸（poly(A+)）RNA的卵母细胞中诱导出大的瞬时内向电流，但在未注射的对照卵母细胞中仅引起小的反应。葡萄糖和果糖在注射了RNA的卵母细胞中也产生内向电流。这些氨基酸的N-甲基化化合物或D-异构体在肉蝇糖受体细胞中诱导的反应与L-氨基酸相比，要么没有反应，要么反应小得多。卵母细胞表达系统是表征味觉转导机制的有用工具。
