Akoev G N, Chalisova N I, Ludino M I, Terent'ev D A, Yatsuk S L, Romanjuk A V
I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, Laboratory of Physiology of Sensory Receptors, St Petersburg, Russia.
Neuroscience. 1996 Nov;75(2):601-5. doi: 10.1016/0306-4522(96)00208-4.
A neurite-stimulating effect was induced by both the cerebrospinal fluid of epileptic patients and the media of co-cultures of rat hippocampus and chick embryo sensory neurons after veratridine treatment. Cerebrospinal fluid from patients with epilepsy stimulated extensive neurite growth in the organotypic culture of chick embryo dorsal root ganglia. The anti-nerve growth factor antibody partly blocked the neurite-stimulating effect of the cerebrospinal fluid. Co-cultures of newborn rat hippocampus and chick embryo dorsal root ganglia were used to investigate the involvement of neurotrophic factors into the processes which are activated by neuronal activity. The data obtained suggest that veratridine, an epileptiform agent, gave rise to an elevation in the level of neurotrophic factors in the culture media and neurite outgrowth of dorsal root ganglia sensory neurons. The anti-nerve growth factor antibody was shown to block the neurite-stimulating effect mediated by veratridine. These results indicate that the epileptiform activity of neurons evokes the expression of neurotrophins.
癫痫患者的脑脊液以及藜芦碱处理后的大鼠海马体与鸡胚感觉神经元共培养物的培养基均诱导出了神经突刺激效应。癫痫患者的脑脊液在鸡胚背根神经节的器官型培养中刺激了广泛的神经突生长。抗神经生长因子抗体部分阻断了脑脊液的神经突刺激效应。新生大鼠海马体与鸡胚背根神经节的共培养用于研究神经营养因子参与由神经元活动激活的过程。所获得的数据表明，藜芦碱这种癫痫样剂导致培养基中神经营养因子水平升高以及背根神经节感觉神经元的神经突生长。抗神经生长因子抗体被证明可阻断藜芦碱介导的神经突刺激效应。这些结果表明，神经元的癫痫样活动引发了神经营养因子的表达。
