Walker D W, Lee N, Heaton M B, King M A, Hunter B E
Gainesville Veterans Administration Medical Center, FL.
Neurosci Lett. 1992 Nov 23;147(1):77-80. doi: 10.1016/0304-3940(92)90778-6.
The effect of chronic ethanol treatment (CET) for 21-26 weeks on the neurotrophic activity contained in the rat hippocampus (HPC) was determined with a bioassay in cultures of dissociated dorsal root ganglion cells (DRG) obtained from E7-8 chick embryos. Extracts of the HPC from CET or pair-fed control rats were used as experimental media, and neuronal survival and neurite-outgrowth of DRG cultures were determined. Both neuronal survival (-25%) and neurite-outgrowth (-50%) were reduced in the presence of HPC extracts from CET rats relative to controls. These data suggest that CET reduces the neurotrophic content of the HPC which may result in damage to septohippocampal neurons.
采用生物测定法，以从E7 - 8日龄鸡胚获取的背根神经节细胞（DRG）解离培养物为实验对象，测定了大鼠海马体（HPC）中含有的神经营养活性受21 - 26周慢性乙醇处理（CET）的影响。将CET大鼠或配对饲养的对照大鼠的HPC提取物用作实验培养基，测定DRG培养物中的神经元存活情况和神经突生长情况。相对于对照组，在存在CET大鼠的HPC提取物的情况下，神经元存活（-25%）和神经突生长（-50%）均减少。这些数据表明，CET降低了HPC的神经营养成分，这可能导致隔海马神经元受损。
