Vogt G
Department of Zoology 1 (Morphology/Ecology), University of Heidelberg, Germany.
J Invertebr Pathol. 1996 Nov;68(3):239-45. doi: 10.1006/jipa.1996.0091.
A new picorna-like or parvo-like virus was found in the hepatopancreas of the shrimp Palaemon elegans from the Bay of Piran (Mediterranean Sea). Bay of Piran shrimp virus (BPSV) infects preferably the R-cells of the hepatopancreas and to a lesser extent the F-cells. In R-cells it is abundant in the cytoplasm and within membrane whorls. In lesser numbers it can also be present in cell organelles like the endoplasmic reticulum, autophagosomes, or mitochondria. Less contrasting virus-like particles are observed in the nucleoplasm, suggesting a nuclear replication of the virus and completion of assembly in the cytoplasm. In F-cells the virus is located mostly in cisternae of the Golgi bodies. Occasionally, there are also virus clusters in the cytoplasm. B-cells are only rarely infected. The virus seems to invade the host cells from the lumen of the hepatopancreatic tubules through the microvillous border. Mature viruses are released again into the hepatopancreatic lumen by discharge of lytic epithelial cells. Individual viruses can also penetrate through the basal cell membrane into the hemal space. Occasionally, fixed phagocytes are attached to the basal lamina of heavily infected hepatopancreas cells, indicating an activation of the immune defense system by the virus.
在皮兰湾（地中海）的秀丽白虾肝胰腺中发现了一种新的类小核糖核酸病毒或细小病毒样病毒。皮兰湾虾病毒（BPSV）主要感染肝胰腺的R细胞，对F细胞的感染程度较轻。在R细胞中，它在细胞质和膜性涡旋中大量存在。数量较少时，它也可存在于内质网、自噬体或线粒体等细胞器中。在核质中观察到对比度较低的病毒样颗粒，提示病毒在细胞核内复制并在细胞质中完成组装。在F细胞中，病毒主要位于高尔基体的扁平囊内。偶尔，细胞质中也有病毒簇。B细胞很少被感染。病毒似乎从肝胰腺小管腔通过微绒毛边界侵入宿主细胞。成熟病毒通过溶解上皮细胞的排出再次释放到肝胰腺管腔中。单个病毒也可穿过基底细胞膜进入血腔。偶尔，固定的吞噬细胞附着在严重感染的肝胰腺细胞的基膜上，表明病毒激活了免疫防御系统。
