flaD（sinR）突变在枯草芽孢杆菌的多个环节影响依赖于SigD的功能。
flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.
作者信息
Rashid M H, Sekiguchi J
机构信息
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
出版信息
J Bacteriol. 1996 Nov;178(22):6640-3. doi: 10.1128/jb.178.22.6640-6643.1996.
Abstract
A flaD (sinR) null mutation depressed sigD-lacZ expression only two- to fourfold, whereas a flaD1 point mutation depressed it almost completely. Introduction of pHYSigD, a sigmaD-overproducing plasmid, corrected the filamentous phenotype common to both sinR mutants; autolysin synthesis was restored partially and completely in the flaD1 and flaD (sinR) null strains, respectively. Flagellin synthesis and motility were not restored at all in either strain.
摘要
flaD（sinR）基因敲除突变使sigD - lacZ表达仅降低2至4倍，而flaD1点突变几乎完全抑制了其表达。引入sigmaD过量表达质粒pHYSigD可纠正两种sinR突变体共有的丝状表型；自溶素合成在flaD1和flaD（sinR）基因敲除菌株中分别部分和完全恢复。两种菌株的鞭毛蛋白合成和运动性均未恢复。
相似文献
1
flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.flaD（sinR）突变在枯草芽孢杆菌的多个环节影响依赖于SigD的功能。
J Bacteriol. 1996 Nov;178(22):6640-3. doi: 10.1128/jb.178.22.6640-6643.1996.
2
Effects of mecA and mecB (clpC) mutations on expression of sigD, which encodes an alternative sigma factor, and autolysin operons and on flagellin synthesis in Bacillus subtilis.mecA和mecB（clpC）突变对枯草芽孢杆菌中编码替代σ因子的sigD、自溶素操纵子的表达以及鞭毛蛋白合成的影响。
J Bacteriol. 1996 Aug;178(16):4861-9. doi: 10.1128/jb.178.16.4861-4869.1996.
3
Effect of the SinR protein on the expression of the Bacillus subtilis 168 lytABC operon.SinR蛋白对枯草芽孢杆菌168 lytABC操纵子表达的影响。
Microb Drug Resist. 1996 Spring;2(1):119-21. doi: 10.1089/mdr.1996.2.119.
4
High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation.枯草芽孢杆菌主要自溶素操纵子的高水平转录依赖于σD基因的表达，并受sin(flaD)突变的影响。
J Bacteriol. 1993 Feb;175(3):795-801. doi: 10.1128/jb.175.3.795-801.1993.
5
FlgM is a primary regulator of sigmaD activity, and its absence restores motility to a sinR mutant.FlgM是σD活性的主要调节因子，其缺失可恢复sinR突变体的运动能力。
J Bacteriol. 1996 Dec;178(23):7010-3. doi: 10.1128/jb.178.23.7010-7013.1996.
6
Environmental regulation of Bacillus subtilis sigma(D)-dependent gene expression.枯草芽孢杆菌σ(D)依赖性基因表达的环境调控
J Bacteriol. 2000 Jun;182(11):3055-62. doi: 10.1128/JB.182.11.3055-3062.2000.
7
Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis.degS-degU突变对枯草芽孢杆菌中编码替代σ因子的sigD及自溶素操纵子表达的影响。
J Bacteriol. 1994 Aug;176(16):5177-80. doi: 10.1128/jb.176.16.5177-5180.1994.
8
Characterization of Bacillus subtilis mutants resistant to cold shock-induced autolysis.枯草芽孢杆菌抗冷休克诱导自溶突变体的特性分析
FEMS Microbiol Lett. 1997 May 15;150(2):269-75. doi: 10.1111/j.1574-6968.1997.tb10380.x.
9
Synthesis of the sigmaD protein is not sufficient to trigger expression of motility functions in Bacillus subtilis.在枯草芽孢杆菌中，σD蛋白的合成不足以触发运动功能的表达。
J Bacteriol. 1999 May;181(9):2942-6. doi: 10.1128/JB.181.9.2942-2946.1999.
10
Role of FlgM in sigma D-dependent gene expression in Bacillus subtilis.FlgM在枯草芽孢杆菌中σD依赖性基因表达中的作用。
J Bacteriol. 1996 Jun;178(11):3113-8. doi: 10.1128/jb.178.11.3113-3118.1996.
引用本文的文献
1
The structure and regulation of flagella in Bacillus subtilis.枯草芽孢杆菌中鞭毛的结构与调控
Annu Rev Genet. 2014;48:319-40. doi: 10.1146/annurev-genet-120213-092406. Epub 2014 Sep 10.
2
Thinking about Bacillus subtilis as a multicellular organism.将枯草芽孢杆菌视为一种多细胞生物。
Curr Opin Microbiol. 2007 Dec;10(6):638-43. doi: 10.1016/j.mib.2007.09.006. Epub 2007 Oct 30.
3
Suppression of engulfment defects in bacillus subtilis by elevated expression of the motility regulon.通过运动调节子的高表达抑制枯草芽孢杆菌中的吞噬缺陷。
J Bacteriol. 2006 Feb;188(3):1159-64. doi: 10.1128/JB.188.3.1159-1164.2006.
4
Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis.枯草芽孢杆菌稳定期σ因子（σ-H）调控子的全基因组分析。
J Bacteriol. 2002 Sep;184(17):4881-90. doi: 10.1128/JB.184.17.4881-4890.2002.
5
Correlation between Bacillus subtilis scoC phenotype and gene expression determined using microarrays for transcriptome analysis.使用微阵列进行转录组分析确定枯草芽孢杆菌scoC表型与基因表达之间的相关性。
J Bacteriol. 2001 Dec;183(24):7329-40. doi: 10.1128/JB.183.24.7329-7340.2001.
6
The transcriptional profile of early to middle sporulation in Bacillus subtilis.枯草芽孢杆菌早期至中期孢子形成的转录谱
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):8063-8. doi: 10.1073/pnas.140209597.
7
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.肽聚糖水解酶LytF在枯草芽孢杆菌营养生长期间与CwlF共同参与细胞分裂过程。
J Bacteriol. 1999 May;181(10):3178-84. doi: 10.1128/JB.181.10.3178-3184.1999.
8
Synthesis of the sigmaD protein is not sufficient to trigger expression of motility functions in Bacillus subtilis.在枯草芽孢杆菌中，σD蛋白的合成不足以触发运动功能的表达。
J Bacteriol. 1999 May;181(9):2942-6. doi: 10.1128/JB.181.9.2942-2946.1999.
9
A molecular switch controlling competence and motility: competence regulatory factors ComS, MecA, and ComK control sigmaD-dependent gene expression in Bacillus subtilis.一种控制感受态和运动性的分子开关：感受态调节因子ComS、MecA和ComK控制枯草芽孢杆菌中依赖于σD的基因表达。
J Bacteriol. 1998 Aug;180(16):4243-51. doi: 10.1128/JB.180.16.4243-4251.1998.
本文引用的文献
1
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌转化的要求。
J Bacteriol. 1961 May;81(5):741-6. doi: 10.1128/jb.81.5.741-746.1961.
2
Effects of mecA and mecB (clpC) mutations on expression of sigD, which encodes an alternative sigma factor, and autolysin operons and on flagellin synthesis in Bacillus subtilis.mecA和mecB（clpC）突变对枯草芽孢杆菌中编码替代σ因子的sigD、自溶素操纵子的表达以及鞭毛蛋白合成的影响。
J Bacteriol. 1996 Aug;178(16):4861-9. doi: 10.1128/jb.178.16.4861-4869.1996.
3
SinI modulates the activity of SinR, a developmental switch protein of Bacillus subtilis, by protein-protein interaction.SinI通过蛋白质-蛋白质相互作用调节枯草芽孢杆菌的发育开关蛋白SinR的活性。
Genes Dev. 1993 Jan;7(1):139-48. doi: 10.1101/gad.7.1.139.
4
Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility.缺乏主要自溶酰胺酶和氨基葡萄糖苷酶的枯草芽孢杆菌突变体在运动能力方面存在缺陷。
FEMS Microbiol Lett. 1993 Sep 1;112(2):135-40. doi: 10.1111/j.1574-6968.1993.tb06438.x.
5
The Bacillus subtilis sigma D-dependent operon encoding the flagellar proteins FliD, FliS, and FliT.编码鞭毛蛋白FliD、FliS和FliT的枯草芽孢杆菌σD依赖性操纵子。
J Bacteriol. 1994 Jun;176(11):3093-101. doi: 10.1128/jb.176.11.3093-3101.1994.
6
Regulation of sigma D expression and activity by spo0, abrB, and sin gene products in Bacillus subtilis.枯草芽孢杆菌中spo0、abrB和sin基因产物对σD表达及活性的调控
J Bacteriol. 1994 Apr;176(8):2435-8. doi: 10.1128/jb.176.8.2435-2438.1994.
7
Characterization of the sigD transcription unit of Bacillus subtilis.枯草芽孢杆菌sigD转录单元的特性分析
J Bacteriol. 1994 Apr;176(8):2427-34. doi: 10.1128/jb.176.8.2427-2434.1994.
8
High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation.枯草芽孢杆菌主要自溶素操纵子的高水平转录依赖于σD基因的表达，并受sin(flaD)突变的影响。
J Bacteriol. 1993 Feb;175(3):795-801. doi: 10.1128/jb.175.3.795-801.1993.
9
Coupling of flagellin gene transcription to flagellar assembly in Bacillus subtilis.枯草芽孢杆菌中鞭毛蛋白基因转录与鞭毛组装的偶联
J Bacteriol. 1994 Aug;176(15):4558-64. doi: 10.1128/jb.176.15.4558-4564.1994.
10
Promoter architecture in the flagellar regulon of Bacillus subtilis: high-level expression of flagellin by the sigma D RNA polymerase requires an upstream promoter element.枯草芽孢杆菌鞭毛调节子中的启动子结构：σD RNA聚合酶驱动鞭毛蛋白的高水平表达需要一个上游启动子元件。
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2582-6. doi: 10.1073/pnas.92.7.2582.
Zotero 插件