FlgM是σD活性的主要调节因子，其缺失可恢复sinR突变体的运动能力。
FlgM is a primary regulator of sigmaD activity, and its absence restores motility to a sinR mutant.
作者信息
Fredrick K, Helmann J D
机构信息
Section of Microbiology, Cornell University, Ithaca, New York 14853, USA.
出版信息
J Bacteriol. 1996 Dec;178(23):7010-3. doi: 10.1128/jb.178.23.7010-7013.1996.
Abstract
We have used mini-Tn1O mutagenesis to identify negative regulators of sigmaD activity. Nine independent insertions were mapped to five genes: flgM, flgK, fliD, fliS, and fliT, suggesting that FlgM export is regulated similarly in Bacillus subtilis and Salmonella typhimurium. We show that a deletion of flgM can restore sigmaD activity to a sinR null mutant of B. subtilis, although fla/che operon expression is affected by neither SinR nor FlgM.
摘要
我们利用mini-Tn1O诱变来鉴定σD活性的负调控因子。九个独立插入位点被定位到五个基因：flgM、flgK、fliD、fliS和fliT，这表明枯草芽孢杆菌和鼠伤寒沙门氏菌中FlgM的输出调控方式相似。我们发现，flgM的缺失可使枯草芽孢杆菌的sinR缺失突变体恢复σD活性，尽管fla/che操纵子的表达不受SinR和FlgM的影响。
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本文引用的文献
1
Role of FlgM in sigma D-dependent gene expression in Bacillus subtilis.FlgM在枯草芽孢杆菌中σD依赖性基因表达中的作用。
J Bacteriol. 1996 Jun;178(11):3113-8. doi: 10.1128/jb.178.11.3113-3118.1996.
2
Molecular dissection of the flagellum-specific anti-sigma factor, FlgM, of Salmonella typhimurium.鼠伤寒沙门氏菌鞭毛特异性抗σ因子FlgM的分子剖析
Mol Gen Genet. 1995 Dec 10;249(4):417-24. doi: 10.1007/BF00287103.
3
Negative regulation by fliD, fliS, and fliT of the export of the flagellum-specific anti-sigma factor, FlgM, in Salmonella typhimurium.鼠伤寒沙门氏菌中鞭毛特异性抗σ因子FlgM输出的fliD、fliS和fliT负调控
J Bacteriol. 1996 Feb;178(3):899-901. doi: 10.1128/jb.178.3.899-901.1996.
4
Transcription of Bacillus subtilis degR is sigma D dependent and suppressed by multicopy proB through sigma D.枯草芽孢杆菌degR的转录依赖于σD，并通过σD被多拷贝proB抑制。
J Bacteriol. 1996 Jan;178(1):216-22. doi: 10.1128/jb.178.1.216-222.1996.
5
Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon.解除枯草芽孢杆菌二肽通透酶操纵子营养阻遏的突变
J Bacteriol. 1993 Aug;175(15):4605-14. doi: 10.1128/jb.175.15.4605-4614.1993.
6
Sensing structural intermediates in bacterial flagellar assembly by export of a negative regulator.通过输出负调控因子来感知细菌鞭毛组装中的结构中间体。
Science. 1993 Nov 19;262(5137):1277-80. doi: 10.1126/science.8235660.
7
The Bacillus subtilis sigma D-dependent operon encoding the flagellar proteins FliD, FliS, and FliT.编码鞭毛蛋白FliD、FliS和FliT的枯草芽孢杆菌σD依赖性操纵子。
J Bacteriol. 1994 Jun;176(11):3093-101. doi: 10.1128/jb.176.11.3093-3101.1994.
8
Regulation of sigma D expression and activity by spo0, abrB, and sin gene products in Bacillus subtilis.枯草芽孢杆菌中spo0、abrB和sin基因产物对σD表达及活性的调控
J Bacteriol. 1994 Apr;176(8):2435-8. doi: 10.1128/jb.176.8.2435-2438.1994.
9
Characterization of the sigD transcription unit of Bacillus subtilis.枯草芽孢杆菌sigD转录单元的特性分析
J Bacteriol. 1994 Apr;176(8):2427-34. doi: 10.1128/jb.176.8.2427-2434.1994.
10
Easy cloning of mini-Tn10 insertions from the Bacillus subtilis chromosome.从小枯草芽孢杆菌染色体中轻松克隆mini-Tn10插入片段。
J Bacteriol. 1994 Mar;176(6):1761-3. doi: 10.1128/jb.176.6.1761-1763.1994.
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