Kreissig S, Schüddekopf K, Dear N, Boehm T
Deutsches Krebforschungszentrum, Heidelberg, Germany.
Nucleic Acids Res. 1996 Nov 1;24(21):4358-9. doi: 10.1093/nar/24.21.4358.
New synthetic approaches, such as combinatorial chemistry, provide a rich source of potential drug candidates. At the same time, the human genome initiative and other large-scale sequencing projects provide a large number of novel drug targets. However, the functional analysis of thousands of new genes remains a major challenge for the future. A systematic strategy for genome-wide functional analysis of genes could employ the fact that at least some modules in multi-domain proteins are encoded in individual exons. Exon amplification provides information about coding regions of most genes that is independent of their transcriptional status; exon amplification from entire mammalian genomes has been demonstrated. Here, we describe the development of an exon-trap system, lambdaGEE (for genomic exon expression), that couples exon amplification with the expression of exon-encoded peptides.
新的合成方法，如组合化学，提供了丰富的潜在药物候选物来源。与此同时，人类基因组计划和其他大规模测序项目提供了大量新的药物靶点。然而，对数千个新基因的功能分析仍是未来的一项重大挑战。一种用于全基因组基因功能分析的系统策略可以利用这样一个事实，即多结构域蛋白中的至少一些模块是由单个外显子编码的。外显子扩增提供了关于大多数基因编码区的信息，这些信息与其转录状态无关；已经证明可以从整个哺乳动物基因组中进行外显子扩增。在这里，我们描述了一种外显子捕获系统lambdaGEE（基因组外显子表达）的开发，该系统将外显子扩增与外显子编码肽的表达相结合。
