Collins R G, Musasche V W, Howley E T
J Appl Physiol Respir Environ Exerc Physiol. 1977 Jul;43(1):164-6. doi: 10.1152/jappl.1977.43.1.164.
Scholander's method of gas analysis requires that the solutions for CO2 absorber, O2 absorber, and acid-rinse be matched in terms of water vapor tension throughout the analysis. Any difference in vapor pressure between either or both of the absorbing solutions and the indicator drop (composed of acid-rinse) will produce a measurable volume change which cannot be attributed to the presence of absorbable gases. This paper describes a practical and quantitative method for preparing reagents whose vapor pressures are matched. A fixed acid-rinse formulation was used throughout. A CO2 absorber prepared from 1.35 N KOH and an O2 absorber prepared from 0.76 N KOH were both matched in terms of vapor pressure with Scholander's acid-rinse solution. Analysis of atmospheric air provided a check on the accuracy of the technique. The values obtained were O2 20.94%, CO2 0.03%, and N2 (balance) 79.04%.
斯科兰德气体分析法要求在整个分析过程中，用于二氧化碳吸收剂、氧气吸收剂和酸洗液的溶液在水蒸气张力方面相互匹配。任何一种或两种吸收溶液与指示剂液滴（由酸洗液组成）之间的蒸气压差异都会产生可测量的体积变化，而这种变化不能归因于可吸收气体的存在。本文描述了一种制备蒸气压相匹配的试剂的实用定量方法。整个过程使用固定的酸洗液配方。由1.35N氢氧化钾制备的二氧化碳吸收剂和由0.76N氢氧化钾制备的氧气吸收剂在蒸气压方面均与斯科兰德酸洗液溶液相匹配。对大气的分析检验了该技术的准确性。得到的值为氧气20.94%、二氧化碳0.03%、氮气（余量）79.04%。
