Lenhoff S, Olofsson T
Department of Medicine, University Hospital, Lund, Sweden.
Cytokine. 1996 Sep;8(9):702-9. doi: 10.1006/cyto.1996.0093.
The cytokine regulation of granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF secretion by human umbilical cord vein endothelial cells (HUVEC) using quantitative immunoassays was studied. Unstimulated HUVEC produced no CSF. Interleukin 1 (IL-1), TNF and lipopolysaccharides (LPS) had stimulatory effects, with IL-1 being the most potent. GM-CSF and G-CSF secretion followed the same pattern, except that more GM-CSF was secreted. Exposure to stimuli for 30 min induced secretion, and detectable amounts in supernatants were found after 4 h incubation. CSF secretion was strictly regulated by the presence of a stimulus in a concentration dependent manner, and there were no signs of any endogenous downregulatory mechanism. No other cytokine tested had any stimulatory effect of its own. However, addition of IL-3 to stimulated HUVEC enhanced both GM-CSF and G-CSF secretion in a dose-dependent manner. In addition, TNF, and to a lesser degree LPS, enhanced IL-1-induced secretion. The only cytokine with a prominent downregulatory effect was IFN-gamma. IL-4 and IL-10, which downregulate CSF secretion by monocytes, had only minor effects.
采用定量免疫分析法研究了人脐静脉内皮细胞（HUVEC）对粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）分泌的细胞因子调节作用。未受刺激的HUVEC不产生集落刺激因子（CSF）。白细胞介素1（IL-1）、肿瘤坏死因子（TNF）和脂多糖（LPS）具有刺激作用，其中IL-1的作用最强。GM-CSF和G-CSF的分泌模式相同，只是GM-CSF的分泌量更多。刺激30分钟可诱导分泌，孵育4小时后在上清液中可检测到。CSF的分泌严格受刺激物浓度的调节，且未发现任何内源性下调机制的迹象。所测试的其他细胞因子自身均无刺激作用。然而，向受刺激的HUVEC中添加IL-3可剂量依赖性地增强GM-CSF和G-CSF的分泌。此外，TNF以及程度较轻的LPS可增强IL-1诱导的分泌。唯一具有显著下调作用的细胞因子是γ干扰素（IFN-γ）。IL-4和IL-10可下调单核细胞的CSF分泌，但作用较小。
