Lenhoff S, Sallerfors B, Olofsson T
Department of Internal Medicine, University Hospital, Lund, Sweden.
Exp Hematol. 1998 Apr;26(4):299-304.
In previous studies of endogenous granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) production, we found several differences in the secretion pattern within and between different cell systems; for example, CSF secretion by endothelial cells is not affected by any major downregulatory factors, whereas monocyte CSF secretion is modulated by several mechanisms. In this study, we characterized the factors that inhibit CSF secretion by monocytes. Three cytokines have inhibitory effects: interleukin (IL)-4, IL-10, and IL-13. Among these, IL-4 and IL-10 have higher potency than IL-13. IL-4 and IL-13 affect GM-CSF and G-CSF secretion to the same extent. In contrast, exogenously added IL-10 has a stronger inhibitory effect on GM-CSF secretion than on G-CSF secretion. We also found that monocytes produce IL-10 with an autocrine downregulatory effect, and that this autocrine IL-10 reaches concentrations at which in most cases only GM-CSF (not G-CSF) secretion is significantly affected. We postulate that the disparate effect of IL-10 on monocyte secretion of the two CSFs reflects their physiological functions, with GM-CSF being mainly a proinflammatory cytokine working in the local compartment and G-CSF functioning mainly as a cell recruiting factor.
在先前关于内源性粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）产生的研究中，我们发现不同细胞系统内部以及之间的分泌模式存在一些差异；例如，内皮细胞分泌CSF不受任何主要下调因子的影响，而单核细胞CSF的分泌则受多种机制调节。在本研究中，我们对抑制单核细胞分泌CSF的因子进行了表征。三种细胞因子具有抑制作用：白细胞介素（IL）-4、IL-10和IL-13。其中，IL-4和IL-10的效力高于IL-13。IL-4和IL-13对GM-CSF和G-CSF分泌的影响程度相同。相比之下，外源性添加的IL-10对GM-CSF分泌的抑制作用比对G-CSF分泌的抑制作用更强。我们还发现单核细胞产生具有自分泌下调作用的IL-10，并且这种自分泌的IL-10达到的浓度在大多数情况下仅显著影响GM-CSF（而非G-CSF）的分泌。我们推测IL-10对单核细胞分泌两种CSF的不同作用反映了它们的生理功能，GM-CSF主要是一种在局部发挥作用的促炎细胞因子，而G-CSF主要作为一种细胞募集因子发挥作用。
