Bohanon T, Elender G, Knoll W, Köberle P, Lee J S, Offenhäusser A, Ringsdorf H, Sackmann E, Simon J, Tovar G, Winnik F M
Institut für organische Chemie, Gutenberg Universität, Mainz, Germany.
J Biomater Sci Polym Ed. 1996;8(1):19-39. doi: 10.1163/156856297x00551.
Control over the adsorption of proteins and over the adsorption and spatial orientation of mammalian cells onto surfaces has been achieved by modification of glass and other silicon oxide substrates with poly(N-isopropylacrylamide) (PNIPAM). The functionalization of the substrates was achieved either by a polymer-analogous reaction of aminosilanes with reactive N-(isopropylacrylamide) (NIPAM)-copolymers and by copolymerization of NIPAM with surface-bound methacrylsilane. The obtained coatings were characterized by FT-1R, ellipsometry, and surface plasmon resonance measurements. The adsorption of two proteins-fibrinogen and ribonuclease A-on these surfaces was studied in situ by real time surface plasmon resonance measurements. The PNIPAM-grafted surfaces prepared by either chemical procedure inhibited the adsorption of both proteins. More importantly they prevented the adhesion of neuroblastomaXglioma hybrid cells cultured either in serum-free medium or in a medium containing serum proteins. Deep-UV irradiation was used to perform ablation processes and to create patterns permitting the examination of spatially controlled adhesion and growth of cells. This study showed that patterned ultrathin polymer films on glass are suitable substrates for controlling the interactions of cells with surfaces and are capable of directing the attachment and spreading of cells.
通过用聚（N-异丙基丙烯酰胺）（PNIPAM）修饰玻璃和其他氧化硅基底，实现了对蛋白质吸附以及哺乳动物细胞在表面的吸附和空间取向的控制。基底的功能化通过氨基硅烷与反应性N-（异丙基丙烯酰胺）（NIPAM）共聚物的聚合物类似反应以及NIPAM与表面结合的甲基丙烯酰硅烷的共聚来实现。通过傅里叶变换红外光谱（FT-1R）、椭偏仪和表面等离子体共振测量对所得涂层进行了表征。通过实时表面等离子体共振测量原位研究了两种蛋白质——纤维蛋白原和核糖核酸酶A在这些表面上的吸附。通过任何一种化学方法制备的PNIPAM接枝表面都抑制了这两种蛋白质的吸附。更重要的是，它们阻止了在无血清培养基或含有血清蛋白的培养基中培养的神经母细胞瘤-胶质瘤杂交细胞的粘附。利用深紫外辐射进行烧蚀过程并创建图案，以允许检查细胞的空间控制粘附和生长。这项研究表明，玻璃上的图案化超薄聚合物膜是用于控制细胞与表面相互作用的合适基底，并且能够指导细胞的附着和铺展。
