Atkins T W, Peacock J
Department of Pharmaceutical and Biological Sciences, Aston University, Birmingham, UK.
J Microencapsul. 1996 Nov-Dec;13(6):709-17. doi: 10.3109/02652049609026054.
Spherical microporous reservoir type microcapsules composed of P(HB-HV) (10.8% HV)/20% PCL containing BSA (surrogate protein) loaded agarose have been fabricated using a double emulsion technique with solvent evaporation. Microcapsules were generated in high yield (> 75 wt%) and BSA incorporation had no significant effect on microcapsule size distribution (21-200 microns). The loss of BSA both by partitioning into the aqueous continuous phase, and through micropores as BSA-loaded-agarose during the precipitation of the fabrication polymer concomitant with solvent evaporation, resulted in low encapsulation efficiency (12%). The amount and duration of BSA release was influenced as much by micropore numbers and diameter as by the extent of reservoir loading and detectable levels of BSA release could be monitored for up to 24 days.
采用溶剂蒸发双乳液技术制备了由P(HB-HV)(10.8% HV)/20% PCL组成的球形微孔储库型微胶囊，其中含有负载牛血清白蛋白（替代蛋白）的琼脂糖。微胶囊的产率很高（> 75 wt%），牛血清白蛋白的掺入对微胶囊的尺寸分布（21 - 200微米）没有显著影响。在制备聚合物沉淀并伴随溶剂蒸发过程中，牛血清白蛋白通过分配到水连续相中以及作为负载牛血清白蛋白的琼脂糖通过微孔而损失，导致包封效率较低（12%）。牛血清白蛋白释放的量和持续时间受微孔数量和直径的影响与储库负载程度的影响一样大，并且可以监测到可检测水平的牛血清白蛋白释放长达24天。
