正常和原发性开角型青光眼中近小管组织的糖胺聚糖分层

Glycosaminoglycan stratification of the juxtacanalicular tissue in normal and primary open-angle glaucoma.

作者信息

Knepper P A, Goossens W, Palmberg P F

机构信息

Laboratory for Oculo-Cerebrospinal Investigation, Children's Memorial Medical Center, Chicago, Illinois, USA.

出版信息

Invest Ophthalmol Vis Sci. 1996 Nov;37(12):2414-25.

PMID:8933758

Abstract

PURPOSE

The juxtacanalicular tissue (JCT) is the probable site of aqueous outflow resistance in normal eyes and of the increased resistance in primary open-angle glaucoma eyes (POAG). The purpose of this histochemical study was to determine the glycosaminoglycan (GAG) composition and stratification in the JCT of POAG and age-matched normal eyes.

METHODS

Five eyes from four normal donors and five eyes from four POAG donors (69 to 80 years of age) were analyzed. Using methods that histochemically preserve GAGs, GAG-degrading enzymes, Alcian blue staining, and real color discrimination to exclude pigment, nuclear staining and unstained areas, the type and amount of GAGs were estimated by compute-raided charge-coupled device color video image analysis. To examine GAG stratification, the JCT was segmented into three regions-anterior, middle, and posterior-to examine regional differences in GAG composition; each region was further divided into four 2-microns layers, from layer 1, adjacent to and including the endothelium of Schlemm's canal, to layer 4, to the first trabecular lamellae.

RESULTS

The normal GAG JCT profile was as follows: hyaluronic acid (HA), 7.78 +/- 1.23 femtograms (fg)/micron2; chondroitin sulfates (CS), 8.18 +/- 0.82 fg/micron2; dermatan sulfate, 0.29 +/- 0.18 fg/micron2; the total, 18.73 +/- 0.68 fg/micron2. In contrast, the POAG GAG JCT profile was as follows: HA 0.57 +/- 0.31 fg/micron2 (P < 0.00001), a 93% decrease; CS 13.49 +/- 0.74 fg/micron2 (P < 0.0001), a 83% increase; dermatan sulfate, 0.90 +/- 0.53 fg/micron2; and the total, 17.31 +/- 0.95 fg/micron2, an 8.2% decrease. The HA was depleted in all layers of all regions of POAG JCT.

CONCLUSIONS

Results indicate that the normal JCT is stratified, with HA as the predominant GAG in layers 1 and 2. The POAG JCT is depleted of HA and has an accumulation of CS, which may increase outflow resistance and, consequently, increase intraocular pressure in patients with POAG.

摘要

目的

在正常眼中，小梁网组织（JCT）可能是房水流出阻力的部位，而在原发性开角型青光眼（POAG）眼中则是阻力增加的部位。本组织化学研究的目的是确定POAG和年龄匹配的正常眼JCT中的糖胺聚糖（GAG）组成和分层情况。

方法

分析了来自4名正常供体的5只眼和来自4名POAG供体（69至80岁）的5只眼。使用组织化学保存GAG、GAG降解酶、阿尔辛蓝染色和真实颜色辨别以排除色素、细胞核染色和未染色区域的方法，通过计算机辅助电荷耦合器件彩色视频图像分析来估计GAG的类型和数量。为了检查GAG分层情况，将JCT分为三个区域——前部、中部和后部——以检查GAG组成的区域差异；每个区域进一步分为四个2微米层，从第1层（与施莱姆管内皮相邻并包括该内皮）到第4层（到第一个小梁薄片）。

结果

正常GAG的JCT分布如下：透明质酸（HA），7.78±1.23飞克（fg）/微米²；硫酸软骨素（CS），8.18±0.82 fg/微米²；硫酸皮肤素，0.29±0.18 fg/微米²；总量，18.73±0.68 fg/微米²。相比之下，POAG的GAG的JCT分布如下：HA 0.57±0.31 fg/微米²（P<0.00001），减少93%；CS 13.49±0.74 fg/微米²（P<0.0001），增加83%；硫酸皮肤素，0.90±0.53 fg/微米²；总量，17.31±0.95 fg/微米²，减少8.2%。POAG的JCT所有区域的所有层中HA均减少。