Romano P, Suzzi G, Brandolini V, Menziani E, Domizio P
Dipartimento di Biologia, Università della Bascilicata, Potenza, Italy.
Lett Appl Microbiol. 1996 Apr;22(4):299-302. doi: 10.1111/j.1472-765x.1996.tb01165.x.
High performance thin layer chromatography with automated multiple development was used to determine 2,3-butanediol levels in wine produced by high and low acetoin-forming strains of Saccharomyces cerevisiae. An inverse correlation between acetoin and 2,3-butanediol content was found suggesting a leaky mutation in acetoin reductase of the low 2,3-butanediol producing strains.
采用自动多次展开的高效薄层色谱法测定了由酿酒酵母中高乙酰乳酸合成菌株和低乙酰乳酸合成菌株所产葡萄酒中的2,3-丁二醇含量。结果发现，乙酰乳酸和2,3-丁二醇含量呈负相关，这表明低2,3-丁二醇产生菌株的乙酰乳酸还原酶存在渗漏突变。
