Khan A A, Wang R F, Cao W W, Franklin W, Cerniglia C E
Microbiology Division, National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079, USA.
Int J Syst Bacteriol. 1996 Apr;46(2):466-9. doi: 10.1099/00207713-46-2-466.
A bacterium isolated from a polluted stream, capable of metabolizing biphenyl, naphthalene, phenanthrene, and higher-molecular-weight polycyclic aromatic hydrocarbons (D. Gibson, V. Mahadevan, D. Jerina, H. Yagi, and H. Yeh, Science 189:295-297, 1975), was previously identified as Beijerinckia sp. strain B1. In this investigation, 16S rRNA gene sequencing, biochemical tests, fatty acid methyl ester analysis, polyacrylamide gel electrophoresis of protein, and DNA-DNA hybridization were used to determine the taxonomic relationship of Beijerinckia sp. strain B1. The sequence of the 16S rRNA gene of B1 was identical to that of Sphingomonas yanoikuyae ATCC 51230T. The biochemical tests, fatty acid analysis, and sodium dodecyl sulfate-polacrylamide gel electrophoresis profile of soluble proteins of strain B1 showed results similar to those of S. yanoikuyae. DNA-DNA hybridization indicated that B1 and S. yanoikuyae ATCC 51230T are 75% homologous at the DNA level. We propose that Beijerinckia sp. strain B1 be reclassified as S. yanoikuyae.
从一条受污染的溪流中分离出的一种细菌，能够代谢联苯、萘、菲和更高分子量的多环芳烃（D. 吉布森、V. 马哈德万、D. 杰里纳、H. 矢木和H. 叶，《科学》189:295 - 297，1975年），先前被鉴定为拜叶林克氏菌属菌株B1。在本研究中，采用16S rRNA基因测序、生化试验、脂肪酸甲酯分析、蛋白质聚丙烯酰胺凝胶电泳以及DNA - DNA杂交来确定拜叶林克氏菌属菌株B1的分类关系。B1的16S rRNA基因序列与矢野鞘氨醇单胞菌ATCC 51230T的序列相同。菌株B1的生化试验、脂肪酸分析以及可溶性蛋白质的十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳图谱显示的结果与矢野鞘氨醇单胞菌的结果相似。DNA - DNA杂交表明，B1与矢野鞘氨醇单胞菌ATCC 51230T在DNA水平上有75%的同源性。我们建议将拜叶林克氏菌属菌株B1重新分类为矢野鞘氨醇单胞菌。
