Matsukawa F
Graduate School of Dentistry, Osaka Dental University, Japan.
J Osaka Dent Univ. 1994 Dec;28(1-2):45-55.
The molecular relationships of extracellular matrix (ECM) from bovine periodontal ligaments to supporting mechanisms of the ligament were investigated. Glycosaminoglycan (GAG) and collagen components of the ECM were compared quantitatively and qualitatively for periodontal tissue adjacent to mandibular anterior (APL) and maxillary posterior (PPL) teeth. There was no significant quantitative difference GAG between the APL and PPL. GAGs in both APL and PPL consisted primarily of dermatan sulfate (DS), chondroitin sulfate (CS) and hyaluronic acid (HA). Heparan sulfate was present as a minor component. Analysis of unsaturated disaccharides obtained from the GAG revealed that delta Di-0S from CS in the PPL was significantly higher than that in the APL, and delta Di-4S from DS in the APL was significantly higher than within the PPL. Furthermore, there was significantly more collagen (as determined by Hyp content) in the APL than in the PPL although the chemical composition of collagen was the same for both. These results suggest that ECM molecules in the APL exhibit both quantitative and qualitative differences to those in the PPL, possibly owing to contrasting mechanisms of tooth support in response to different masticatory functions.
研究了牛牙周韧带细胞外基质（ECM）与韧带支持机制的分子关系。对下颌前牙（APL）和上颌后牙（PPL）相邻牙周组织的ECM中糖胺聚糖（GAG）和胶原蛋白成分进行了定量和定性比较。APL和PPL之间的GAG在数量上没有显著差异。APL和PPL中的GAG主要由硫酸皮肤素（DS）、硫酸软骨素（CS）和透明质酸（HA）组成。硫酸乙酰肝素作为次要成分存在。对从GAG中获得的不饱和二糖的分析表明，PPL中CS的δDi-0S显著高于APL，APL中DS的δDi-4S显著高于PPL。此外，APL中的胶原蛋白（以羟脯氨酸含量测定）明显多于PPL，尽管两者的胶原蛋白化学成分相同。这些结果表明，APL中的ECM分子与PPL中的ECM分子在数量和质量上均存在差异，这可能是由于对不同咀嚼功能的牙齿支持机制不同所致。
