Hamburger K, Kramhoft B, Nissen S B, Zeuthen E
J Cell Sci. 1977 Apr;24:69-79. doi: 10.1242/jcs.24.1.69.
The glycolytic activity of 3 different synchronous system in S. pombe was studied. Synchronous cultures were produced by a selection procedure, by cyclic heat treatment, or by cloning cells from an exponentially multiplying culture. In all experiments a complex medium with 3% glucose was used. The glycolytic activity was recorded with a gasometric method, the gradient diver. A single cell in exponential growth or a small number of synchronized cells were placed in ampulla divers in which the cells progressed undistrubed through a number of cycles. An ampulla diver is in principle a narrow pipette by which a single or a few cells are removed from the mother culture. It serves next as an axenic growth chamber and at the same time as a gasometer. The divers were placed in linear saline density gradients and the gaseous exchanges taking place in the divers resulted in migration of the divers. The migration rate is a measure of the glycolytic activity of the cells. Our results show that the glycolytic activity increases in a linear fashion between sucessive divisions. The rate of increase doubles at each division. This true in all 3 synchronous systems, and we take this as an indication that the cell cycles of heat-synchronized cells do not deviate seriously from the normal.
对粟酒裂殖酵母中3种不同同步系统的糖酵解活性进行了研究。通过选择程序、循环热处理或从指数增殖培养物中克隆细胞来产生同步培养物。在所有实验中，均使用含3%葡萄糖的复杂培养基。用气量法、梯度潜水器记录糖酵解活性。将处于指数生长期的单个细胞或少量同步化细胞置于潜水器安瓿中，细胞在其中不受干扰地经历多个周期。潜水器安瓿原则上是一种细吸管，通过它从母培养物中取出单个或少数细胞。接下来，它用作无菌生长室，同时用作气量计。将潜水器置于线性盐水密度梯度中，潜水器中发生的气体交换导致潜水器迁移。迁移速率是细胞糖酵解活性的一种度量。我们的结果表明，在连续分裂之间，糖酵解活性呈线性增加。每次分裂时增加速率翻倍。在所有3种同步系统中都是如此，我们以此作为热同步化细胞的细胞周期与正常细胞周期没有严重偏差的一个指标。
