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通过一种自我传播载体对极端嗜热嗜酸古菌嗜热栖热菌进行转化。

Transformation of the extremely thermoacidophilic archaeon Sulfolobus solfataricus via a self-spreading vector.

作者信息

Elferink M G, Schleper C, Zillig W

机构信息

Max-Planck-Institut für Biochemie, Martinsried, Germany. elferink/vms.bioc

出版信息

FEMS Microbiol Lett. 1996 Mar 15;137(1):31-5. doi: 10.1111/j.1574-6968.1996.tb08078.x.

DOI:10.1111/j.1574-6968.1996.tb08078.x
PMID:8935654
Abstract

We describe a transformation system for extremely thermophilic archaea of the genus Sulfolobus in the kingdom Crenarchaeota. We have constructed in vitro a recombinant derivative of the recently described conjugative plasmid pNOB8, containing a beta-galactosidase gene downstream of a strong promotor. Transformation of a beta-galactosidase negative mutant of Sulfolobus solfataricus with this construct resulted in its spreading through the culture containing the primary transformants and in efficient restoration of beta-galactosidase activity.

摘要

我们描述了一种用于泉古菌界硫化叶菌属超嗜热古菌的转化系统。我们在体外构建了最近描述的接合质粒pNOB8的重组衍生物，该衍生物在强启动子下游含有一个β-半乳糖苷酶基因。用该构建体转化嗜热栖热硫化叶菌的β-半乳糖苷酶阴性突变体，导致其在含有初级转化体的培养物中扩散，并有效恢复β-半乳糖苷酶活性。

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