生物流体中药物的高压液相色谱分析。IV. 氯贝酸的测定。
High-pressure liquid chromatographic analysis of drugs in biological fluids. IV. Determination of clofibrinic acid.
作者信息
Bjornsson T D, Blaschke T F, Meffin P J
出版信息
J Chromatogr. 1977 Jul 11;137(1):145-52. doi: 10.1016/s0021-9673(00)89249-3.
A rapid, sensitive and specific high-pressure liquid chromatographic method is described for the quantitative analysis of clofibrinic acid in plasma, saliva and urine. In contrast to previously reported gas-liquid chromatographic methods, which require derivatization of clofibrinic acid before chromatography, the present method involves a simple two-step extraction procedure and chromatographic determination of the underivatized clofibrinic acid. Concentrations between 1.0 and 25.0 microgram per sample can be measured with a coefficient of variation from 1 to 6%.
本文描述了一种快速、灵敏且特异的高压液相色谱法，用于定量分析血浆、唾液和尿液中的氯贝酸。与之前报道的气液色谱法不同，后者在色谱分析前需要对氯贝酸进行衍生化处理，而本方法只需简单的两步萃取程序，并对未衍生化的氯贝酸进行色谱测定。每个样品中1.0至25.0微克的浓度范围均可测定，变异系数为1%至6%。
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