一种用于测定血浆和尿液中氯苯氧基异丁酸的快速气相色谱法。
A rapid gas chromatographic method for the determination of chlorophenoxyisobutyric acid in plasma and urine.
作者信息
Gugler R, Jensen C
出版信息
J Chromatogr. 1976 Feb 4;117(1):175-9. doi: 10.1016/s0021-9673(00)81079-1.
A rapid gas chromatographic method is described for the determination of chlorophenoxyisobutyric acid (the active metabolite of clofibrate) in plasma and urine. The assay involves an extraction into toluene and back-extraction of the chlorophenoxyisobutyric acid and the internal standard (2-naphthoic acid) into the methylating reagent (trimethylanilinium hydroxide). Concentrations of 1 mug/ml in plasma and urine can easily be measured; the precision of the method is 3.3 +/- 0.7% for plasma and 2.7 +/- 0.4% for urine. There is no interference from endogenous compounds or from drugs commonly prescribed together with clofibrate.
本文描述了一种快速气相色谱法，用于测定血浆和尿液中的氯苯氧异丁酸（氯贝丁酯的活性代谢物）。该测定方法包括用甲苯萃取，然后将氯苯氧异丁酸和内标物（2-萘甲酸）反萃取到甲基化试剂（氢氧化三甲基苯胺）中。血浆和尿液中1微克/毫升的浓度很容易测定；该方法的精密度对于血浆为3.3±0.7%，对于尿液为2.7±0.4%。内源性化合物或与氯贝丁酯一起常用的药物均无干扰。
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